Department of Medical Sciences, Uppsala University, Uppsala, Sweden; CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Dr. António Bernardino Almeida, Porto, 4200-072, Portugal.
Environ Microbiol. 2014 Apr;16(4):995-1004. doi: 10.1111/1462-2920.12231. Epub 2013 Aug 27.
The influence of geographic distribution and type of habitat on the molecular epidemiology of ciprofloxacin resistant Escherichia coli was investigated. Ciprofloxacin resistant E. coli from wastewater, urban water with faecal contamination and faeces of gulls, pigeons and birds of prey, from Portugal, Spain and Sweden were compared based on multi-locus sequence typing (MLST) and quinolone resistance genetic determinants. Multi-locus sequence typing allowed the differentiation of E. coli lineages associated with birds of prey from those inhabiting gulls and waters. E. coli lineages of clinical relevance, such as the complex ST131, were detected in wastewater, streams and gulls in Portugal, Spain and Sweden. Quinolone resistance was due to gyrA and parC mutations, although distinct mutations were detected in birds of prey and in wastewater, streams and gulls isolates. These differences were correlated with specific MLST lineages, suggesting resistance inheritance. Among the plasmid-mediated quinolone resistance genes, only aac(6')-ib-cr and qnrS were detected in wastewater, streams and gulls isolates, but not in birds of prey. The horizontal transfer of the gene aac(6')-ib-cr could be inferred from its occurrence in different MLST lineages.
本研究旨在探讨地理分布和生境类型对环丙沙星耐药大肠杆菌分子流行病学的影响。对来自葡萄牙、西班牙和瑞典的污水、受粪便污染的城市水以及海鸥、鸽子和猛禽粪便中的环丙沙星耐药大肠杆菌进行了比较，比较的依据是多位点序列分型（MLST）和喹诺酮耐药遗传决定因素。多位点序列分型可区分与猛禽相关的大肠杆菌谱系与栖息在海鸥和水域中的大肠杆菌谱系。在葡萄牙、西班牙和瑞典的废水中、溪流中和海鸥中检测到了与临床相关的大肠杆菌谱系，如复杂的 ST131。喹诺酮耐药是由于 gyraA 和 parC 突变引起的，尽管在猛禽中和在废水、溪流和海鸥分离株中检测到了不同的突变。这些差异与特定的 MLST 谱系相关，提示存在耐药性遗传。在质粒介导的喹诺酮耐药基因中，仅在废水中、溪流和海鸥分离株中检测到 aac(6')-ib-cr 和 qnrS，而在猛禽中未检测到。基因 aac(6')-ib-cr 的水平转移可从其在不同 MLST 谱系中的存在推断出来。
