Merkeviciene Lina, Ruzauskaite Neda, Klimiene Irena, Siugzdiniene Rita, Dailidaviciene Jurgita, Virgailis Marius, Mockeliunas Raimundas, Ruzauskas Modestas
Lithuanian University of Health Sciences, LT-44307 Kaunas, Lithuania.
J Vet Res. 2017 Apr 4;61(1):27-35. doi: 10.1515/jvetres-2017-0004. eCollection 2017 Mar.
The aim of the study was to determine microbiota in the cloacal samples of European herring gulls () and to compare a variety of genes encoding antimicrobial resistance in cultivable and non-cultivable bacteria.
Cloacal samples from European herring gulls were collected from a Kaunas city dump. Cultivable microbiota were isolated, their microbial susceptibility was tested, and genes encoding antimicrobial resistance were detected. Additionally, a metagenomic study was performed using Next-Generation Sequencing (NGS).
In total, 697 different operational taxonomic units at genus level were detected; however, only 63 taxonomic units were detected at the amount of ≥0.1% of the total number of DNA copies. was found to have the highest prevalence. The bacterial amount of other genera was up to 5% with the most highly prevalent being (4.7%), (4.5%), unclassified Enterococcaceae (3.2%), (2.9%), and (2.6%).
are predominant microbiota in the cloacal samples of . This species of gulls is a reservoir of bacteria carrying a wide-spectrum of genes encoding antimicrobial resistance. The same genes were detected in both cultivable microbiota and in the total DNA of the samples.
本研究的目的是确定欧洲银鸥泄殖腔样本中的微生物群，并比较可培养和不可培养细菌中多种编码抗菌抗性的基因。
从考纳斯市垃圾场采集欧洲银鸥的泄殖腔样本。分离可培养的微生物群，测试其微生物敏感性，并检测编码抗菌抗性的基因。此外，使用下一代测序（NGS）进行宏基因组研究。
总共检测到697个属水平的不同操作分类单元；然而，仅检测到63个分类单元，其数量占DNA拷贝总数的≥0.1%。发现[具体属名]的患病率最高。其他属的细菌数量高达5%，其中最普遍的是[具体属名]（4.7%）、[具体属名]（4.5%）、未分类的肠球菌科（3.2%）、[具体属名]（2.9%）和[具体属名]（2.6%）。
[具体属名]是欧洲银鸥泄殖腔样本中的主要微生物群。这种鸥类是携带多种编码抗菌抗性基因的细菌的储存库。在可培养微生物群和样本的总DNA中都检测到了相同的基因。
