Cell- and Neurobiology, Zoological Institute, Haid-und-Neu-Strasse 9, 76131 Karlsruhe, Germany and Institute of Functional Interfaces (IFG), Karlsruhe Institute of Technology (KIT) Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
Adv Mater. 2013 Nov 13;25(42):6117-22. doi: 10.1002/adma.201302678. Epub 2013 Sep 4.
Spatial control over the surface chemistry of 3D organic-inorganic hybrid microscaffolds is achieved by a two-photon-triggered cycloaddition. Following a coating step with photoactivatable dienes via silanization, surface irradiation with a femtosecond-pulsed laser in the presence of functional dienophiles enables a site-selective alteration of the surface chemistry. Bioconjugation with fluorescent protein tags is employed to reveal the 3D molecular patterns.
通过双光子引发的环加成反应实现了对 3D 有机-无机杂化微支架表面化学性质的空间控制。通过硅烷化作用用可光活化的二烯进行涂层步骤后，在存在官能化二烯的情况下用飞秒脉冲激光进行表面辐照，可实现对表面化学性质的选择性改变。使用带有荧光蛋白标签的生物偶联来揭示 3D 分子图案。
