Gupta Kamal K, Pal Namrata, Mishra Pradeep K, Srivastava Pradeep, Mohanty Sujata, Maiti Pralay
Department of Chemical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi-221005, India.
J Biomed Mater Res A. 2014 Aug;102(8):2600-12. doi: 10.1002/jbm.a.34932. Epub 2013 Sep 3.
Hybrid nanofibers of poly(lactic acid) and polycaprolactone have been developed by embedding cancerous drug through electrospinning technique. The composition of polymer has been varied to check the compositional effect on properties. The quality of nanofibers has been testified through surface morphology, wetting properties using contact angle and mechanical strength under uniaxial elongation. The compatibility of drug (5-fluorourasil) with matrix fiber has been verified using Fourier transform infrared, X-ray diffraction, Raman spectroscopy, and differential scanning calorimetry. The drug release study has been performed showing greater release in hybrid fibers when compared with pure polymers as a result of synergism of two immiscible polymers and quasi-Fickian diffusion mechanism in hybrid nanofiber as implants showing compositional effect on drug release. A model has been proposed showing faster release of drugs in hybrid systems. Biological responses through fluorescence imaging and MTT assay confirm the release of drug from hybrid nanofibers showing potential use of hybrid scaffolds as chemotherapeutic implant.
通过静电纺丝技术将抗癌药物包埋其中，制备了聚乳酸和聚己内酯的混合纳米纤维。改变聚合物的组成以研究其对性能的影响。通过表面形态、使用接触角的润湿性以及单轴拉伸下的机械强度来验证纳米纤维的质量。使用傅里叶变换红外光谱、X射线衍射、拉曼光谱和差示扫描量热法验证了药物（5-氟尿嘧啶）与基质纤维的相容性。进行了药物释放研究，结果表明，由于两种不相容聚合物的协同作用以及混合纳米纤维中的准菲克扩散机制，与纯聚合物相比，混合纤维中的药物释放量更大，作为植入物的混合纳米纤维显示出组成对药物释放的影响。提出了一个模型，表明混合系统中药物释放更快。通过荧光成像和MTT分析的生物学反应证实了混合纳米纤维中药物的释放，表明混合支架作为化疗植入物具有潜在用途。
