Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA.
Infect Immun. 2013 Dec;81(12):4363-76. doi: 10.1128/IAI.00819-13. Epub 2013 Sep 16.
The potent phagocytic and microbicidal activities of neutrophils and macrophages are among the first lines of defense against bacterial infections. Yet Staphylococcus aureus is often resistant to innate immune defense mechanisms, especially when organized as a biofilm. To investigate how S. aureus biofilms respond to macrophages and neutrophils, gene expression patterns were profiled using Affymetrix microarrays. The addition of macrophages to S. aureus static biofilms led to a global suppression of the biofilm transcriptome with a wide variety of genes downregulated. Notably, genes involved in metabolism, cell wall synthesis/structure, and transcription/translation/replication were among the most highly downregulated, which was most dramatic at 1 h compared to 24 h following macrophage addition to biofilms. Unexpectedly, few genes were enhanced in biofilms after macrophage challenge. Unlike coculture with macrophages, coculture of S. aureus static biofilms with neutrophils did not greatly influence the biofilm transcriptome. Collectively, these experiments demonstrate that S. aureus biofilms differentially modify their gene expression patterns depending on the leukocyte subset encountered.
中性粒细胞和巨噬细胞的吞噬和杀菌活性是抵御细菌感染的第一道防线。然而，金黄色葡萄球菌通常对先天免疫防御机制具有抗性，特别是当其形成生物膜时。为了研究金黄色葡萄球菌生物膜如何对巨噬细胞和中性粒细胞做出反应，我们使用 Affymetrix 微阵列对基因表达模式进行了分析。将巨噬细胞添加到金黄色葡萄球菌静态生物膜中会导致生物膜转录组的全局抑制，大量基因下调。值得注意的是，参与代谢、细胞壁合成/结构以及转录/翻译/复制的基因是下调最明显的基因，与巨噬细胞添加到生物膜后 1 小时相比，24 小时时更为显著。出乎意料的是，在巨噬细胞挑战后，生物膜中的许多基因并没有增强。与与巨噬细胞共培养不同，金黄色葡萄球菌静态生物膜与中性粒细胞的共培养对生物膜转录组的影响不大。总的来说，这些实验表明，金黄色葡萄球菌生物膜根据所遇到的白细胞亚群来差异化地修饰其基因表达模式。
