Cao Thanh V, Hicks Michael R, Campbell David, Standley Paul R
Research Specialist, Department of Basic Medical Sciences, The University of Arizona College of Medicine-Phoenix, Phoenix, AZ.
J Manipulative Physiol Ther. 2013 Oct;36(8):513-21. doi: 10.1016/j.jmpt.2013.07.004. Epub 2013 Sep 16.
The purpose of this study was to investigate potential differences of magnitudes and durations associated with dosed myofascial release (MFR) on human fibroblast proliferation, hypertrophy, and cytokine secretions.
Bioengineered tendons (BETs) attached to nylon mesh anchors were strained uniaxially using a vacuum pressure designed to model MFR varying in magnitudes (0%, 3%, 6%, 9%, and 12% elongation) and durations (0.5 and 1-5 minutes). Conditioned media were analyzed for cytokine secretion via protein microarray (n = 2). Bioengineered tendons were weighted and fibroblasts extracted from the BET were assessed for total cell protein and proliferation via double-stranded DNA quantification (n = 5). All data were compared by a 1-way analysis of variance with post hoc Dunnett test and Student t test.
Changing MFR magnitude and duration did not have an effect on total fibroblast cellular protein or DNA accumulation. However, we observed a stepwise increase in BET weight with higher-magnitude MFR treatments. Longer durations of MFR resulted in progressive increase in the secretions of angiogenin, interleukin (IL)-3, IL-8, growth colony-stimulating factor, and thymus activation-regulated chemokine. Alternatively, increasing strain magnitude induced secretions of IL-1β, monocyte chemoattractant cytokine, and regulated and normal T cell expressed and secreted chemotactic cytokine.
Cellular proliferation and hypertrophy were not significantly changed by any treatment. However, the change in total BET dry weight suggests that production of extracellular matrix protein may be up-regulated. Different MFR parameters induce secretions of a unique subset of cytokines and growth factors that can be further enhanced by increasing the magnitude and duration of treatment. If clinically translatable, these results suggest that variations to manual therapy biomechanical parameters may differentially affect physiological responses in vivo.
本研究旨在调查定量肌筋膜松解术（MFR）在人体成纤维细胞增殖、肥大及细胞因子分泌方面的幅度和持续时间的潜在差异。
将附着于尼龙网锚的生物工程肌腱通过真空压力进行单轴拉伸，该真空压力旨在模拟不同幅度（0%、3%、6%、9%和12%伸长）和持续时间（0.5分钟以及1 - 5分钟）的MFR。通过蛋白质微阵列分析条件培养基中的细胞因子分泌情况（n = 2）。对生物工程肌腱进行称重，并通过双链DNA定量评估从生物工程肌腱中提取的成纤维细胞的总细胞蛋白和增殖情况（n = 5）。所有数据通过单因素方差分析以及事后Dunnett检验和学生t检验进行比较。
改变MFR的幅度和持续时间对成纤维细胞总细胞蛋白或DNA积累没有影响。然而，我们观察到随着较高幅度的MFR治疗，生物工程肌腱重量呈逐步增加。MFR持续时间延长导致血管生成素、白细胞介素（IL）-3、IL-8、生长集落刺激因子和胸腺激活调节趋化因子的分泌逐渐增加。另外，应变幅度增加诱导IL-1β、单核细胞趋化因子以及调节性和正常T细胞表达和分泌的趋化因子的分泌。
任何治疗均未显著改变细胞增殖和肥大。然而，生物工程肌腱总干重的变化表明细胞外基质蛋白的产生可能被上调。不同的MFR参数诱导独特的细胞因子和生长因子亚群的分泌，通过增加治疗的幅度和持续时间可进一步增强。如果能转化至临床，这些结果表明手动治疗生物力学参数的变化可能会对体内生理反应产生不同影响。
