Ahmad Manzoor, Suhail Nida, Mansoor Tariq, Banu Naheed, Ahmad Shamshad
Department of General Surgery, J.N. Medical College, A.M.U., Aligarh, India.
Indian J Clin Biochem. 2012 Oct;27(4):385-8. doi: 10.1007/s12291-012-0229-4. Epub 2012 Jun 12.
In the present study, oxidative stress and lymphocytic DNA damage in both pre-op and post-op benign prostrate hyperplasia (BPH) patients with age >50 years was evaluated and compared with normal healthy subjects (controls- without any evidence of disease) of the same sex and age group. From December 2007 to November 2009, oxidative stress in 45 BPH patients were evaluated both before (pre-op patients) and after 7 days of surgery (post-op patients) in terms of measurements of plasma levels of (1) various anti-oxidative enzymes, (2) non-enzymatic antioxidants and (3) malondialdehyde which is a product of lipid peroxidation. The lymphocyte DNA damage was also evaluated by single cell alkaline gel electrophoresis in terms of tail length migration in these patients. These values were compared with their respective control subjects of similar sex and age group. The activities of antioxidant enzymes and the levels of antioxidant, reduced glutathione were found significantly decreased (p < 0.05) in serum samples of pre-operative group of BPH patients as compared to the controls. These altered parameters increased significantly (p < 0.05) and returned to their near normal control values, but not up to baseline values, in post operative patients i.e. after the cancer load was decreased by surgery. Lymphocytic DNA damage was found to be significantly increased in pre-op group as compared to controls and was reduced after surgery in post-op group. The present study therefore, shows significantly increased levels of oxidative stress and DNA damage in BPH patients which were reduced after removal of tumour load. Thus oxidative damage plays an important role in prostate tumourogenesis and timely management of oxidative stress can be of importance in preventing the occurrence of BPH.
在本研究中，对年龄大于50岁的术前和术后良性前列腺增生（BPH）患者的氧化应激和淋巴细胞DNA损伤进行了评估，并与相同性别和年龄组的正常健康受试者（对照组 - 无任何疾病证据）进行了比较。从2007年12月至2009年11月，对45例BPH患者在手术前（术前患者）和手术后7天（术后患者）的氧化应激进行了评估，评估指标包括：（1）各种抗氧化酶的血浆水平；（2）非酶抗氧化剂；（3）脂质过氧化产物丙二醛。还通过单细胞碱性凝胶电泳根据这些患者的尾长迁移评估淋巴细胞DNA损伤。将这些值与其相似性别和年龄组的各自对照受试者进行比较。与对照组相比，BPH患者术前组血清样本中的抗氧化酶活性和抗氧化剂还原型谷胱甘肽水平显著降低（p <0.05）。这些改变的参数在术后患者中即手术降低肿瘤负荷后显著增加（p <0.05）并恢复到接近正常对照值，但未达到基线值。与对照组相比，术前组淋巴细胞DNA损伤显著增加，术后组手术后减少。因此，本研究表明BPH患者的氧化应激水平和DNA损伤显著增加，在去除肿瘤负荷后降低。因此，氧化损伤在前列腺肿瘤发生中起重要作用，及时管理氧化应激对于预防BPH的发生可能很重要。
