State Key Laboratory of Chemical Resource Engineering, School of Science, Beijing University of Chemical Technology , Beijing 100029, China.
ACS Appl Mater Interfaces. 2013 Nov 13;5(21):10502-9. doi: 10.1021/am4020605. Epub 2013 Oct 18.
A facile method is presented for the selective luminescence detection of trace polycyclic aromatic hydrocarbons (PAHs) based on a combination of the specific recognition of molecularly imprinted polymers (MIPs) and magnetic separation (MS). Multifunctional magnetic-luminescent MIP nanocomposites were fabricated via a one-pot emulsion strategy using polystyrene-co-methacrylic acid copolymer, hydrophobic Fe3O4 nanoparticles and luminescent LaVO4:Eu(3+) nanoparticles as building blocks with a phenanthrene template. The resulting nanocomposites can be employed in a simple method for the luminescence detection of phenanthrene. Furthermore, magnetic separation of the nanocomposites from the target mixture prior to luminescence detection of phenanthrene affords significantly enhanced selectivity and sensitivity, with a 3σ limit of detection (LOD) as low as 3.64 ng/mL. Milk samples spiked with phenanthrene (5.0 μg/mL) were assayed via this method and recoveries ranging from 97.11 to 101.9% were obtained, showing that our strategy is potentially applicable for the preconcentration, recovering, and monitoring of trace PAHs in complex mixtures.
本文提出了一种基于分子印迹聚合物（MIPs）的特异性识别和磁分离（MS）相结合的简便方法，用于痕量多环芳烃（PAHs）的选择性荧光检测。通过一锅乳液策略，使用聚苯乙烯-甲基丙烯酸共聚物、疏水性 Fe3O4 纳米粒子和发光 LaVO4:Eu（3+）纳米粒子作为构建块，并以菲为模板，制备了多功能磁性发光 MIP 纳米复合材料。所得纳米复合材料可用于简单的荧光检测方法，用于菲的检测。此外，在荧光检测菲之前，通过磁分离纳米复合材料从目标混合物中分离，可显著提高选择性和灵敏度，其检测限（LOD）低至 3.64ng/mL。通过该方法测定牛奶样品中菲的含量（5.0μg/mL），回收率在 97.11%至 101.9%之间，表明该策略在复杂混合物中痕量 PAHs 的预浓缩、回收和监测方面具有潜在的应用前景。
