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革兰氏染色、DNA探针及培养法在女性生殖道标本中鉴定动弯杆菌属菌种的比较

Comparison of gram stain, DNA probe, and culture for the identification of species of Mobiluncus in female genital specimens.

作者信息

Roberts M C, Hillier S L, Schoenknecht F D, Holmes K K

出版信息

J Infect Dis. 1985 Jul;152(1):74-7. doi: 10.1093/infdis/152.1.74.

DOI:10.1093/infdis/152.1.74
PMID:2409187
Abstract

The detection of species of Mobiluncus in female genital specimens by DNA probe (with whole-chromosomal bacterial DNA), culture, and gram stain were compared by using specimens obtained from hospital patients, college students, and women attending a sexually transmitted disease clinic. Culture purification and speciation required an average of 37 days to complete, whereas the DNA-probe assay required five days. Gram stain was also rapid but did not allow speciation. There was 100% correlation between species identification by DNA probe and by conventional biochemical tests. Gram stain alone detected 90% of the samples that were positive by any method, whereas culture detected 77%-83% and DNA probe detected 52%-83%.

摘要

通过DNA探针(使用全染色体细菌DNA)、培养和革兰氏染色,对从医院患者、大学生和就诊于性传播疾病诊所的女性中获取的女性生殖器标本中动弯杆菌属的菌种进行了检测比较。培养纯化和菌种鉴定平均需要37天才能完成,而DNA探针检测只需5天。革兰氏染色也很快,但无法进行菌种鉴定。DNA探针鉴定的菌种与传统生化测试鉴定的菌种之间存在100%的相关性。仅革兰氏染色检测出通过任何方法呈阳性的样本中的90%,而培养法检测出77%-83%,DNA探针检测出52%-83%。

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