Department of Life Science and Chemical Engineering, Hunan University of Science and Engineering, Yongzhou 425100, PR China.
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, 178 Daxue Road, Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2014 Feb;64(Pt 2):444-448. doi: 10.1099/ijs.0.050229-0. Epub 2013 Oct 4.
A Gram-negative, oxidase- and catalase-positive, rod-shaped, non-spore-forming, motile, aerobic bacterium, designated Y2(T), was isolated from surface seawater of Yundang Lake, Xiamen, China. The strain was able to grow in the presence of 0.5-6.0% NaCl (optimum 1.0-1.5%), at pH 5-10 (optimum pH 8) and at 10-40 °C (optimum 25 °C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Y2(T) belongs to the genus Pseudoalteromonas, with the highest sequence similarity of 94.9% to Pseudoalteromonas tunicata D2(T); within the genus Pseudoalteromonas, it showed the lowest similarity of 92.8% to Pseudoalteromonas denitrificans ATCC 43337(T). The G+C content of the chromosomal DNA of strain Y2(T) was 45.1 mol%. The predominant fatty acids were summed feature 3 (C(16 : 1)ω6c and/or C(16 : 1)ω7c), C(16 : 0), C(12 : 0) 3-OH and summed feature 8 (C(18 : 1)ω6c and/or C(18 : 1)ω7c). The only respiratory quinone detected was Q-8. Based on the phylogenetic and phenotypic characteristics, strain Y2(T) represents a novel species of the genus Pseudoalteromonas, for which the name Pseudoalteromonas xiamenensis sp. nov. is proposed; the type strain is Y2(T) ( = CGMCC 1.12157(T) = JCM 18779(T)).
一株革兰氏阴性、氧化酶和接触酶阳性、杆状、非孢子形成、运动、需氧细菌，被命名为 Y2(T)，是从中国厦门云顶湖的表层海水中分离得到的。该菌株能够在 0.5-6.0%NaCl（最佳 1.0-1.5%）、pH5-10（最佳 pH8）和 10-40°C（最佳 25°C）的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 Y2(T)属于假交替单胞菌属，与假交替单胞菌 D2(T)的序列相似性最高为 94.9%；在假交替单胞菌属中，与假交替单胞菌 D2(T)的序列相似性最低为 92.8%。菌株 Y2(T)的染色体 DNA 的 G+C 含量为 45.1mol%。主要脂肪酸为 sumed feature 3（C(16 : 1)ω6c 和/或 C(16 : 1)ω7c）、C(16 : 0）、C(12 : 0) 3-OH 和 summed feature 8（C(18 : 1)ω6c 和/或 C(18 : 1)ω7c）。唯一检测到的呼吸醌是 Q-8。基于系统发育和表型特征，菌株 Y2(T)代表假交替单胞菌属的一个新种，现命名为假交替单胞菌厦门亚种 sp. nov.；模式菌株为 Y2(T)（=CGMCC 1.12157(T)=JCM 18779(T)）。
