Cruz Andreia, Moreira Rafael, Mendo Sónia
Departamento de Biologia & CESAM, Universidade de Aveiro, Campus de Santiago, 3810-193, Aveiro, Portugal,
Folia Microbiol (Praha). 2014 May;59(3):203-7. doi: 10.1007/s12223-013-0284-0. Epub 2013 Oct 5.
Tributyltin (TBT) contamination remains a major problem worldwide. Many laboratories are committed to the development of remediation methodologies that could help reduce the negative impact of this compound in the environment. Furthermore, it is important to have at hand simple methodologies for evaluating TBT toxicity in the laboratory, besides the use of complex and costly analytical instrumentation. With that purpose, a method was adapted that is based on the inhibition of growth of an indicator strain, Micrococcus luteus ATCC 9341, under TBT. Different types of matrices, of TBT concentrations and sample treatments were tested. The results herein reported show that the bioassay method can be applied for both aqueous and soil samples and also for a high range of TBT concentrations (at least up to 500 μmol/L). Besides being cheap and easy to perform, it can be performed in any laboratory. Additionally, one possible application of the method to monitor TBT degradation is presented as an example.
三丁基锡（TBT）污染仍然是一个全球性的主要问题。许多实验室致力于开发能够帮助减少这种化合物对环境负面影响的修复方法。此外，除了使用复杂且昂贵的分析仪器外，拥有简单的实验室评估TBT毒性的方法也很重要。出于这个目的，采用了一种基于在TBT作用下抑制指示菌株藤黄微球菌ATCC 9341生长的方法。测试了不同类型的基质、TBT浓度和样品处理。本文报道的结果表明，该生物测定方法可应用于水样和土壤样品，也可用于高范围的TBT浓度（至少高达500μmol/L）。该方法不仅便宜且易于操作，而且可以在任何实验室进行。此外，还举例说明了该方法在监测TBT降解方面的一种可能应用。
