Chaves Thiago P, Fernandes Felipe Hugo A, Santana Cleildo P, Santos Jocimar S, Medeiros Francinalva D, Felismino Délcio C, Santos Vanda L, Catão Raïssa Mayer R, Coutinho Henrique Douglas M, Medeiros Ana Cláudia D
Laboratory of drug assay and development, Universidade Estadual da Paraíba, Campina Grande, Paraíba, Brazil.
Department of Natural Sciences, Universidade Federal do Piauí, Bom Jesus, Piauí, Brazil.
PLoS One. 2016 May 18;11(5):e0155532. doi: 10.1371/journal.pone.0155532. eCollection 2016.
Poincianella pyramidalis (Tul.) LP Queiroz (Fabaceae) is an endemic tree of northeastern Brazil, occurring mainly in the Caatinga. Its medicinal use is widespread and is an important therapeutic option against diarrhea, dysentery, and respiratory and urinary infections, among other diseases. In this study we determined the chemical marker and evaluated the interaction between P. pyramidalis extract and a commercial antimicrobial through the use of biological and analytical models. To obtain the extract, an ethanol-water mixture (50:50 v/v) was used as solvent. It was nebulized in a spray dryer using colloidal silicon dioxide as a drying adjuvant. The extract (ENPp) was subjected to HPLC analysis to verify the presence of certain secondary metabolites. The Minimum Inhibitory Concentration (MIC) of the extract against Gram-negative bacteria was determined by broth microdilution and the MIC of synthetic antimicrobial drugs in the presence and absence of the extract. The antioxidant activity of ENPp was evaluated by the DPPH method. The compatibility between the antimicrobial and the extract was evaluated by thermal analysis (TG/DTA). The acute toxicity of the extract was evaluated in vivo in rodents. The results indicate significant additive action of the extract on synthetic antibiotics, considerable antioxidant activity and absence of toxicity. This extract shows high potential for the development of formulations for antimicrobial therapy when used with a vegetable-active ingredient.
金字塔叶决明（Poincianella pyramidalis (Tul.) LP Queiroz）（豆科）是巴西东北部的一种特有树种，主要生长在卡廷加地区。其药用广泛，是治疗腹泻、痢疾以及呼吸道和泌尿系统感染等疾病的重要治疗选择。在本研究中，我们通过生物和分析模型确定了化学标志物，并评估了金字塔叶决明提取物与一种商业抗菌剂之间的相互作用。为了获得提取物，使用乙醇 - 水混合物（50:50 v/v）作为溶剂。在喷雾干燥器中使用胶体二氧化硅作为干燥助剂对其进行喷雾干燥。对提取物（ENPp）进行高效液相色谱分析以验证某些次生代谢产物的存在。通过肉汤微量稀释法测定提取物对革兰氏阴性菌的最低抑菌浓度（MIC）以及在有和没有提取物存在的情况下合成抗菌药物的MIC。通过DPPH法评估ENPp的抗氧化活性。通过热分析（TG/DTA）评估抗菌剂与提取物之间的相容性。在啮齿动物体内评估提取物的急性毒性。结果表明提取物对合成抗生素有显著的相加作用，具有相当的抗氧化活性且无毒性。当与植物活性成分一起使用时，这种提取物在开发抗菌治疗制剂方面具有很高的潜力。
