Department of Pharmacy, Sunchon National University, Suncheon 540-742.
Biomol Ther (Seoul). 2012 Mar;20(2):201-6. doi: 10.4062/biomolther.2012.20.2.201.
Ultraviolet (UV) A penetrates deeply into the skin and induces the generation of reactive oxygen species (ROS) causing damage to fibroblasts, which leads to aging of the skin. However, the body has developed an antioxidant defence system against the harmful effects of ROS. Enzymes such as superoxide dismutase (SOD) and catalase (CAT) play critical roles on the removal of excess ROS in living organisms. In this study, the antioxidant activities of anthocyanins (cyanidin 3-galactoside and cyanidin 3-lathyroside) from Acanthopanax divaricatus var. albeofructus (ADA) fruits were investigated by xylenol orange, thiobarbituric acid reactive substances (TBARS), and antioxidant enzyme assay. As a result, generation of H2O2 and lipid peroxide induced by UVA-irradiation in human dermal fibroblast (HDF-N) cells was reduced by treatment of anthocyanins. Also, augmented enzyme (SOD and CAT) activities were observed in UVA-irradiated cells when treated with anthocyanin. In conclusion, the results obtained show that anthocyanins from ADA fruits are potential candidates for the protection of fibroblast against the damaging effects of UVA irradiation. Furthermore, anthocyanin may be a good candidate for antioxidant agent development.
紫外线 (UV) A 可深入穿透皮肤，并诱导活性氧 (ROS) 的生成，导致成纤维细胞损伤，进而导致皮肤衰老。然而，人体已经发展出了一种抗氧化防御系统来对抗 ROS 的有害影响。超氧化物歧化酶 (SOD) 和过氧化氢酶 (CAT) 等酶在清除生物体内过量的 ROS 方面起着关键作用。在这项研究中，通过二甲氧唑橙、硫代巴比妥酸反应物 (TBARS) 和抗氧化酶测定法，研究了五加属植物变叶五加果实中花色苷（矢车菊素 3-半乳糖苷和矢车菊素 3-李糖苷）的抗氧化活性。结果表明，花色苷处理可减少 UVA 辐射诱导的人真皮成纤维细胞 (HDF-N) 中 H2O2 和脂质过氧化物的生成。此外，当用花色苷处理 UVA 辐射的细胞时，观察到增强的酶 (SOD 和 CAT) 活性。综上所述，研究结果表明，变叶五加果实中的花色苷是保护成纤维细胞免受 UVA 辐射损伤的潜在候选物质。此外，花色苷可能是开发抗氧化剂的良好候选物质。
