Alghamdi Hamdan S, van den Beucken Jeroen J J P, Jansen John A
1 Department of Biomaterials, Radboud University Medical Center , Nijmegen, The Netherlands .
Tissue Eng Part C Methods. 2014 Jun;20(6):493-505. doi: 10.1089/ten.TEC.2013.0327. Epub 2013 Nov 26.
Osseointegration of dental and orthopedic bone implants is the important process that leads to mechanical fixation of implants and warrants implant functionality. In view of increasing numbers of osteoporotic patients, bone implant surface optimization strategies with instructive and drug-loading ability have been heavily explored. However, few animal models are available to study the effect of novel implant surface modifications in osteoporotic conditions. Since laboratory rats comply with a number of practical advantages, including the reliability of several methods for rapid induction of osteoporotic conditions, the present work aimed to define the use of the femoral condyle in osteoporotic female and male rats as a suitable implantation model to study osseointegration of bone implants. The method describes the procedures for induction (by hypogonadism) and assessment (by in vivo micro-computed tomography [CT]) of osteoporotic conditions in both female and male rats. The implantation site architecture (femoral condyle bone properties and dimensions) was comparatively evaluated for female and male rats, and the implant installation procedures are described. Finally, the possible analytical techniques to evaluate bone responses via mechanical tests, ex vivo micro-CT, and histological methods are provided.
牙科和骨科骨植入物的骨整合是导致植入物机械固定并确保植入物功能的重要过程。鉴于骨质疏松患者数量不断增加，具有诱导性和药物负载能力的骨植入物表面优化策略已得到大量探索。然而，很少有动物模型可用于研究新型植入物表面修饰在骨质疏松条件下的效果。由于实验大鼠具有许多实际优势，包括多种快速诱导骨质疏松条件方法的可靠性，本研究旨在确定在骨质疏松的雌性和雄性大鼠中使用股骨髁作为研究骨植入物骨整合的合适植入模型。该方法描述了雌性和雄性大鼠骨质疏松条件的诱导（通过性腺功能减退）和评估（通过体内微型计算机断层扫描[CT]）程序。对雌性和雄性大鼠的植入部位结构（股骨髁骨特性和尺寸）进行了比较评估，并描述了植入物安装程序。最后，提供了通过机械测试、离体微型CT和组织学方法评估骨反应的可能分析技术。
