Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
ACS Nano. 2012 Aug 28;6(8):6730-40. doi: 10.1021/nn301389c. Epub 2012 Jul 26.
We form coronas of serum proteins on gold nanorods (NRs) coated with cetyltrimethylammonium bromide (CTAB). These coronas can be exploited for their ability to hold small molecular therapeutics at a capacity much higher (~5-10×) than what covalent conjugation strategies can achieve. Coronas are loaded with DNA oligonucleotides and Doxorubicin, showing that they can hold species of either negative or positive charge. Payload capacity varies with assembly strategy, ionic strength, and loading concentration. Payload release can be achieved by increasing the temperature or by ultrafast laser excitation of the NRs at their longitudinal surface plasmon resonance. DNA leakage from the corona is minimal within the first 3 days of preparation, although Dox leakage was more significant. The coronas also stabilize the NRs in buffer and biological media. This study demonstrates the biological utility of the protein corona around nanomaterials, contrasting the common view of the corona as an undesirable biological response.
我们在包覆十六烷基三甲基溴化铵（CTAB）的金纳米棒（NR）上形成血清蛋白冠。这些蛋白冠可以利用其结合小分子治疗药物的能力，其载药量比共价键合策略高得多（约 5-10 倍）。蛋白冠负载 DNA 寡核苷酸和阿霉素，表明它们可以结合带正电荷或负电荷的物质。载药量随组装策略、离子强度和加载浓度而变化。通过提高温度或通过在纵向表面等离子体共振处对 NR 进行超快激光激发，可以实现药物释放。在制备后的前 3 天内，DNA 从蛋白冠中的泄漏量很小，尽管阿霉素的泄漏量更大。蛋白冠还可以在缓冲液和生物介质中稳定 NR。这项研究证明了纳米材料周围蛋白冠的生物学效用，与将蛋白冠视为不良生物反应的常见观点形成对比。
