Hu Zhengyan, Zhang Hongyan, Zhang Yi, Wu Ren'an, Zou Hanfa
CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian 116023, China.
Colloids Surf B Biointerfaces. 2014 Sep 1;121:354-61. doi: 10.1016/j.colsurfb.2014.06.016. Epub 2014 Jun 12.
When nanoparticles (NPs) enter into biological systems, proteins would interact with NPs to form the protein corona that can critically impact the biological identity of the nanomaterial. Owing to their fundamental scientific interest and potential applications, Fe3O4 NPs of different sizes have been developed for applications in cell separation and protein separation and as contrast agents in magnetic resonance imaging (MRI), etc. Here, we investigated whether nanoparticle size affects the formation of protein coronas around Fe3O4 NPs. Both the identification and quantification results demonstrated that particle size does play an important role in the formation of plasma protein coronas on Fe3O4 NPs; it not only influenced the protein composition of the formed plasma protein corona but also affected the abundances of the plasma proteins within the coronas. Understanding the different binding profiles of human plasma proteins on Fe3O4 NPs of different sizes would facilitate the exploration of the bio-distributions and biological fates of Fe3O4 NPs in biological systems.
当纳米颗粒（NPs）进入生物系统时，蛋白质会与NPs相互作用形成蛋白质冠层，这会对纳米材料的生物学特性产生关键影响。由于其具有基础科学研究价值和潜在应用价值，不同尺寸的Fe3O4纳米颗粒已被开发用于细胞分离、蛋白质分离以及磁共振成像（MRI）中的造影剂等应用。在此，我们研究了纳米颗粒尺寸是否会影响Fe3O4纳米颗粒周围蛋白质冠层的形成。鉴定和定量结果均表明，颗粒尺寸在Fe3O4纳米颗粒表面血浆蛋白质冠层的形成中确实起着重要作用；它不仅影响所形成的血浆蛋白质冠层的蛋白质组成，还影响冠层内血浆蛋白质的丰度。了解人类血浆蛋白质在不同尺寸Fe3O4纳米颗粒上的不同结合情况，将有助于探索Fe3O4纳米颗粒在生物系统中的生物分布和生物学命运。
