从鸡胚肌肉制备多核糖体氨酰转移核糖核酸
Preparation of polyribosome aminoacyl-transfer ribonucleic acid from the muscle of chick embryos.
作者信息
Nwagwu M
出版信息
Biochem J. 1975 Jun;147(3):473-7. doi: 10.1042/bj1470473.
Abstract
A procedure for preparing polyribosome aminoacyl-tRNA free from contamination by supernatant aminoacyl-tRNA and free amino acids is described. Important features of the procedure are the use of acidic buffers to help protect the amino acid-tRNA linkage and the inclusion of sodium dodecyl sulphate, to inhibit ribonuclease activity. The specific radioactivity of polyribosome aminoacyl-tRNA is high within 30s and reaches a maximum in 2 1/2 min, well ahead of polyribosome peptides which, as described by Herrmann et al. (1971), attain maximum specific radioactivity in about 10 min.
摘要
本文描述了一种制备不含上清液氨酰tRNA和游离氨基酸污染的多核糖体氨酰tRNA的方法。该方法的重要特点是使用酸性缓冲液来帮助保护氨基酸-tRNA连接，并加入十二烷基硫酸钠以抑制核糖核酸酶活性。多核糖体氨酰tRNA的比放射性在30秒内就很高，并在2分半钟内达到最大值，这比多核糖体肽要早得多，正如赫尔曼等人（1971年）所描述的，多核糖体肽在大约10分钟内达到最大比放射性。
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