Bechgaard E
Acta Derm Venereol. 1985;65(4):282-6.
An objective method for the quantification of follicular hyperkeratosis is proposed for the rabbit ear assay of acnegenicity. The area (area%) of hyperkeratinized follicles, covering a stereomicroscopic photo of the epidermis, is proposed for the quantification of the response. The uniformity of two observers' quantification was evaluated in six tested formulations. The quantification of the response in area% was found to be a suitable and objective alternative to visual scoring on a graded scale. The median relative difference between two observers' area%-quantification was found to be 26%, and there was no statistically significant difference between the two observers' quantification (p greater than 0.10). An area% of 2, 7, and 15 is preliminarily suggested to be equivalent to the lower limit for scores 1, 2 and 3 respectively.
针对兔耳致粉刺性试验，提出了一种用于量化毛囊角化过度的客观方法。建议通过覆盖表皮体视显微镜照片的角化过度毛囊面积（面积百分比）来量化反应。在六种受试制剂中评估了两名观察者量化结果的一致性。发现以面积百分比量化反应是一种合适且客观的方法，可替代分级视觉评分。两名观察者面积百分比量化结果的中位相对差异为26%，且两名观察者的量化结果之间无统计学显著差异（p大于0.10）。初步建议面积百分比为2、7和15分别相当于评分1、2和3的下限。
