1] Massachusetts General Hospital Center for Regenerative Medicine, 185 Cambridge Street, Boston, Massachusetts 02114, USA. [2] Harvard Stem Cell Institute, 1350 Masschusetts Avenue, Cambridge, Massachusetts 02138, USA. [3] Howard Hughes Medical Institute, 4000 Jones Bridge Road, Chevy Chase, Maryland 20815, USA. [4] Department of Stem Cell and Regenerative Biology, Harvard University and Harvard Medical School, 7 Divinity Avenue, Cambridge, Massachusetts 02138, USA.
Nature. 2013 Oct 24;502(7472):462-71. doi: 10.1038/nature12749.
Induced pluripotency is a powerful tool to derive patient-specific stem cells. In addition, it provides a unique assay to study the interplay between transcription factors and chromatin structure. Here, we review the latest insights into chromatin dynamics that are inherent to induced pluripotency. Moreover, we compare and contrast these events with other physiological and pathological processes that involve changes in chromatin and cell state, including germ cell maturation and tumorigenesis. We propose that an integrated view of these seemingly diverse processes could provide mechanistic insights into cell fate transitions in general and might lead to new approaches in regenerative medicine and cancer treatment.
诱导多能性是一种强大的工具，可以衍生出患者特异性的干细胞。此外，它提供了一种独特的检测方法，用于研究转录因子和染色质结构之间的相互作用。在这里，我们回顾了与诱导多能性相关的染色质动力学的最新见解。此外，我们将这些事件与其他涉及染色质和细胞状态变化的生理和病理过程进行了比较和对比，包括生殖细胞成熟和肿瘤发生。我们提出，对这些看似不同的过程进行综合观察，可以为细胞命运转变提供机制上的见解，并可能为再生医学和癌症治疗带来新的方法。
