博来霉素的放射免疫测定法。

Radioimmunoassay of bleomycins.

作者信息

Seki T, Muraoka Y, Takahashi K, Horinishi H, Umezawa H

出版信息

J Antibiot (Tokyo). 1985 Sep;38(9):1251-6. doi: 10.7164/antibiotics.38.1251.

DOI:10.7164/antibiotics.38.1251

Abstract

Rabbit antisera highly specific to the bleomycinic acid moiety of bleomycins were obtained by immunizing with a conjugate of copper-complex of bleomycin A5 and bovine serum albumin. These antisera not only reacted with bleomycin A5 but also with other bleomycins such as bleomycin A2, bleomycin B2 and peplomycin. The antisera showed little cross-reactivity with deamido-, depyruvamido- and decarbamoyl-bleomycins. Thus, these antisera were found to be highly specific for the intact bleomycinic acid moiety. One of the antisera was successfully applied to radioimmunoassay of bleomycin and peplomycin in mouse and human sera. The detection limit was 1 ng/ml. This radioimmunoassay is expected to be widely used for the determination of active bleomycins in biological and clinical samples.

摘要

通过用博来霉素A5的铜络合物与牛血清白蛋白的缀合物进行免疫，获得了对博来霉素的博来霉素酸部分具有高度特异性的兔抗血清。这些抗血清不仅与博来霉素A5反应，还与其他博来霉素如博来霉素A2、博来霉素B2和培洛霉素反应。该抗血清与脱氨基、脱丙酮酸酰胺基和脱氨甲酰基博来霉素几乎没有交叉反应。因此，发现这些抗血清对完整的博来霉素酸部分具有高度特异性。其中一种抗血清成功应用于小鼠和人血清中博来霉素和培洛霉素的放射免疫测定。检测限为1 ng/ml。这种放射免疫测定有望广泛用于生物和临床样品中活性博来霉素的测定。