Eggink Dirk, Goff Peter H, Palese Peter
Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
J Virol. 2014 Jan;88(1):699-704. doi: 10.1128/JVI.02608-13. Epub 2013 Oct 23.
Influenza virus hemagglutinin consists of a highly variable and immunodominant head domain and a more conserved but immunosubdominant stalk domain. We introduced seven N-linked glycosylation sites in the hemagglutinin head domain to shield the immunodominant antigenic sites. The hyperglycosylated hemagglutinin enhanced stalk-directed seroreactivity while dampening the head response in immunized mice. Upon influenza virus challenge, mice vaccinated with the hyperglycosylated hemagglutinin were better protected against morbidity and mortality than mice receiving the wild-type hemagglutinin.
流感病毒血凝素由一个高度可变且免疫显性的头部结构域和一个更保守但免疫亚显性的茎部结构域组成。我们在血凝素头部结构域引入了七个N - 连接糖基化位点，以屏蔽免疫显性抗原位点。高糖基化的血凝素增强了针对茎部的血清反应性，同时抑制了免疫小鼠中针对头部的反应。在受到流感病毒攻击后，接种高糖基化血凝素的小鼠比接受野生型血凝素的小鼠在预防发病和死亡方面得到了更好的保护。
