Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Kuwait University, The 4th Ring Road, Jabriya, P.O. Box 31470, 90805 Sulaibikhat, Kuwait.
Dis Markers. 2013;35(4):235-41. doi: 10.1155/2013/931915. Epub 2013 Sep 9.
This study evaluates peripheral blood T lymphocyte expression of inflammatory and proinflammatory cytokines as well as T regulatory (Treg) (FOXP3+CD25+CD4+) cells in type 2 diabetes (T2DM). Participants included 40 T2DM and 30 healthy control subjects. Twenty-four patients had no complications while 16 were afflicted with coronary heart disease (CHD). Relative to healthy subjects, all T2DM patients showed a significant increase in expression of CD4+IFN- Y +, CD4+TNF- α +, and CD4+IL-8+ T cells (P < 0.001) as well as CD4+IL-6+, CD4+IL-1 β +, and IL-17+ T cells (P < 0.05) while the ratios of Treg/Th1(CD4+IFN- Y +) and Treg/Th-17(CD4+IL-17+) cells were significantly decreased (P < 0.05 and P < 0.01). T2DM patients with CHD showed a significant increase in CD4+IFN- Y +, CD4+TNF- α +, and CD4+IL-17+ T cells and a significant decrease in Treg/Th1 and Treg/IL-17 cells compared to T2DM patients without CHD (P < 0.05). In CHD-afflicted T2DM, HbA1c correlated positively with CD4+IFN- Y + T cells (P < 0.01), HDL correlated negatively with each of CD4+IL-8+ T cells and CD4+IL-17+ T cells (P < 0.05), and LDL correlated positively with CD4+IL-1 β + T cells (P < 0.05). Conclusion. This study shows that hyperglycemia and dyslipidemia correlate with increased inflammatory cytokine expression and suggests the involvement of T cells in the development of diabetes and its complications.
这项研究评估了 2 型糖尿病（T2DM）患者外周血 T 淋巴细胞中炎症和促炎细胞因子以及 T 调节（Treg）（FOXP3+CD25+CD4+）细胞的表达。参与者包括 40 名 T2DM 患者和 30 名健康对照者。24 名患者无并发症，16 名患者患有冠心病（CHD）。与健康对照组相比，所有 T2DM 患者的 CD4+IFN-γ+、CD4+TNF-α+和 CD4+IL-8+T 细胞（P<0.001）以及 CD4+IL-6+、CD4+IL-1β+和 IL-17+T 细胞（P<0.05）表达显著增加，而 Treg/Th1（CD4+IFN-γ+）和 Treg/Th-17（CD4+IL-17+）细胞的比例显著降低（P<0.05 和 P<0.01）。与无 CHD 的 T2DM 患者相比，患有 CHD 的 T2DM 患者的 CD4+IFN-γ+、CD4+TNF-α+和 CD4+IL-17+T 细胞显著增加，Treg/Th1 和 Treg/IL-17 细胞显著减少（P<0.05）。在患有 CHD 的 T2DM 患者中，HbA1c 与 CD4+IFN-γ+T 细胞呈正相关（P<0.01），HDL 与 CD4+IL-8+T 细胞和 CD4+IL-17+T 细胞呈负相关（P<0.05），而 LDL 与 CD4+IL-1β+T 细胞呈正相关（P<0.05）。结论。本研究表明，高血糖和血脂异常与炎症细胞因子表达增加有关，并提示 T 细胞参与了糖尿病及其并发症的发生。
