Cardiovascular Research Institute, University of California, 94122, San Francisco, California.
J Membr Biol. 1971 Dec;4(1):252-69. doi: 10.1007/BF02431974.
As determined by electron microscopy, lipid sonicated in buffer initially forms large vesicles which may be multilamellar. Prolonged sonication results in a population of vesicles of smaller, but not uniform diameters. These vesicles are bounded by only one bilayer. The lipid suspension can be partially fractionated according to size by column chromatography. A fraction of the eluate has been selected for further study. The weight-average vesicle weight and average radius of gyration are obtained by lightscattering measurements. The volume of buffer enclosed by the vesicles is determined using(14)C- or(3)H-labelled sugars as a marker. These values are in reasonable agreement with the corresponding values calculated from the size distribution of the vesicle fraction obtained by electron microscopy.
通过电子显微镜观察确定，脂质在缓冲液中超声处理后最初形成大的囊泡，可能是多层的。长时间的超声处理会导致囊泡的群体变小，但直径并不均匀。这些囊泡只有一个双层膜。脂质悬浮液可以根据大小通过柱层析进行部分分级。洗脱液的一部分已被选来进行进一步的研究。通过光散射测量得到重量平均囊泡重量和平均回转半径。使用(14)C-或(3)H-标记的糖作为标记物来确定囊泡内封闭的缓冲液体积。这些值与通过电子显微镜获得的囊泡部分的大小分布计算出的相应值吻合较好。
