Plant Genetics Section, Environmental Science and Technology Department, Risø National Laboratory, DK-4000, Roskilde, Denmark.
Theor Appl Genet. 1994 Nov;89(6):676-81. doi: 10.1007/BF00223704.
Genetic variability of cultivated and wild barley, Hordeum vulgare ssp. vulgare and spontaneum, respectively, was assessed by RFLP analysis. The material consisted of 13 European varietes, single-plant offspring lines of eight land races from Ethiopia and Nepal, and five accessions of ssp. spontaneum from Israel, Iran and Turkey. Seventeen out of twenty-one studied cDNA and gDNA probes distributed across all seven barley chromosomes revealed polymorphism when DNA was digested with one of four restriction enzymes. A tree based on genetic distances using frequencies of RFLP banding patterns was estimated and the barley lines clustered into five groups reflecting geographical origin. The geographical groups of land-race lines showed less intragroup variation than the geographical groups of spontaneum lines. The group of European varieties, representing large variation in agronomic traits, showed an intermediate level. The proportion of gene diversity residing among geographical groups (FST) varied from 0.19 to 0.94 (average 0.54) per RFLP pattern, indicating large diversification between geographical groups.
通过 RFLP 分析评估了栽培大麦和野生大麦（分别为 Hordeum vulgare ssp. vulgare 和 spontaneum）的遗传变异性。材料包括 13 个欧洲品种、来自埃塞俄比亚和尼泊尔的 8 个地方品种的单株后代系以及来自以色列、伊朗和土耳其的 5 个 spontaneum 品种。在对四种限制酶消化的 DNA 进行研究时，分布在大麦 7 条染色体上的 21 个 cDNA 和 gDNA 探针中的 17 个探针显示出多态性。使用 RFLP 带型频率估算了基于遗传距离的树，并根据地理起源将大麦系聚类为 5 组。地方品种系的地理群显示出比 spontaneum 系的地理群更少的组内变异。代表农业性状大量变异的欧洲品种组显示出中等水平。基因多样性在地理群体之间的比例（FST）每个 RFLP 模式变化范围为 0.19 至 0.94（平均 0.54），表明地理群体之间存在大量多样化。
