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Validation of routine mycoplasma testing by PCR.

作者信息

Toji L H, Lenchitz T C, Kwiatkowski V A, Sarama J A, Mulivor R A

出版信息

In Vitro Cell Dev Biol Anim. 1998 May;34(5):356-8. doi: 10.1007/s11626-998-0014-0.

DOI:10.1007/s11626-998-0014-0
PMID:9639095
Abstract
摘要

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Validation of a PCR coupled to a microarray method for detection of mycoplasma in vaccines.用于检测疫苗中支原体的聚合酶链反应(PCR)与微阵列方法联用的验证
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Validation of a PCR method for the detection of mycoplasmas according to European Pharmacopoeia section 2.6.7.根据欧洲药典第2.6.7节对用于检测支原体的PCR方法进行验证。
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本文引用的文献

1
Contamination of human cell cultures by pleuropneumonialike organisms.类胸膜肺炎微生物对人类细胞培养物的污染。
Science. 1956 Dec 7;124(3232):1147-8. doi: 10.1126/science.124.3232.1147.
2
Advances in PCR-based detection of mycoplasmas contaminating cell cultures.基于PCR检测污染细胞培养物的支原体的进展。
PCR Methods Appl. 1995 Feb;4(4):199-208. doi: 10.1101/gr.4.4.199.
3
Specificity and sensitivity of polymerase chain reaction (PCR) in comparison with other methods for the detection of mycoplasma contamination in cell lines.
尿路上皮膀胱癌中的滋养层细胞表面抗原2与miR-125b轴
Oncotarget. 2017 Apr 25;8(35):58642-58653. doi: 10.18632/oncotarget.17407. eCollection 2017 Aug 29.
4
tRF-Leu-CAG promotes cell proliferation and cell cycle in non-small cell lung cancer.tRF-Leu-CAG促进非小细胞肺癌中的细胞增殖和细胞周期。
Chem Biol Drug Des. 2017 Nov;90(5):730-738. doi: 10.1111/cbdd.12994. Epub 2017 May 22.
5
Twist2 promotes kidney cancer cell proliferation and invasion by regulating ITGA6 and CD44 expression in the ECM-receptor interaction pathway.Twist2通过调节细胞外基质-受体相互作用途径中的ITGA6和CD44表达来促进肾癌细胞的增殖和侵袭。
Onco Targets Ther. 2016 Mar 29;9:1801-12. doi: 10.2147/OTT.S96535. eCollection 2016.
6
Antitumor activity of a novel oncrasin analogue is mediated by JNK activation and STAT3 inhibition.新型癌抑素类似物通过 JNK 激活和 STAT3 抑制发挥抗肿瘤活性。
PLoS One. 2011;6(12):e28487. doi: 10.1371/journal.pone.0028487. Epub 2011 Dec 12.
7
Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF.源自慢性伤口患者的原代培养成纤维细胞:一种生产人类细胞系并测试如粒细胞-巨噬细胞集落刺激因子等假定生长因子疗法的方法。
J Transl Med. 2008 Dec 1;6:75. doi: 10.1186/1479-5876-6-75.
8
Microbiological control in stem cell banks: approaches to standardisation.干细胞库中的微生物控制:标准化方法
Appl Microbiol Biotechnol. 2005 Sep;68(4):456-66. doi: 10.1007/s00253-005-0062-2. Epub 2005 Oct 26.
9
Comparative PCR analysis for detection of mycoplasma infections in continuous cell lines.用于检测连续细胞系中支原体感染的比较PCR分析
In Vitro Cell Dev Biol Anim. 2002 Feb;38(2):79-85. doi: 10.1290/1071-2690(2002)038<0079:CPAFDO>2.0.CO;2.
与其他检测细胞系支原体污染方法相比,聚合酶链反应(PCR)的特异性和敏感性
J Immunol Methods. 1993 Aug 26;164(1):91-100. doi: 10.1016/0022-1759(93)90279-g.
4
DNA probes and PCR in diagnosis of mycoplasma infections.用于支原体感染诊断的DNA探针与聚合酶链反应
Mol Cell Probes. 1994 Dec;8(6):497-511. doi: 10.1006/mcpr.1994.1071.
5
Cytogenetic effects of mycoplasmal infection of cell cultures: a review.细胞培养支原体感染的细胞遗传学效应:综述
In Vitro. 1984 Jan;20(1):1-18. doi: 10.1007/BF02633326.
6
A review of cell culture contaminations.细胞培养污染综述。
In Vitro. 1971 Jul-Aug;7(1):26-41. doi: 10.1007/BF02619002.
7
Comparative studies to determine the efficiency of 6 methylpurine deoxyriboside to detect cell culture mycoplasmas.比较研究以确定6-甲基嘌呤脱氧核糖苷检测细胞培养支原体的效率。
In Vitro Cell Dev Biol. 1986 Jun;22(6):301-4. doi: 10.1007/BF02623401.
8
Detection of cell culture mycoplasmas by a genetic probe.用基因探针检测细胞培养中的支原体
Exp Cell Res. 1986 Mar;163(1):273-8. doi: 10.1016/0014-4827(86)90581-1.
9
Comparative studies between microbiological culture and uptake of uridine/uracil to detect mycoplasmal infection of cell cultures.
Exp Cell Res. 1979 Jun;121(1):159-65. doi: 10.1016/0014-4827(79)90456-7.