通过三体分析结合等位基因间互补对酿酒酵母中arg1和arg8进行基因定位。
Genetic mapping of arg1 and arg8 in Saccharomyces cerevisiae by trisomic analysis combined with interallelic complementation.
作者信息
Hilger F, Mortimer R K
出版信息
J Bacteriol. 1980 Jan;141(1):270-4. doi: 10.1128/jb.141.1.270-274.1980.
Abstract
Through use of multiply disomic strains, the genes arg1 and arg8 were excluded from all of chromosomes I to XVII except (i) XV and (ii) IX and XV, respectively. Further aneuploid analyses showed that these two genes were on the same chromosome. By tetrad analysis, arg1 was shown to be linked to SUP3 on the left arm of chromosome XV (parental ditype:nonparental ditype:tetratype = 74; 6:139) and arg8 was shown to be loosely linked to arg1 (parental ditype:nonparental ditype:tetratype 72:17:220) on the same arm. The sequence of the genes on this chromosome arm is centromere-SUP3-arg8. Because arg1 had previously been used to define an 18th chromosome, these results reestablished the minimum chromosome number in Saccharomyces cerevisiae as 17.
摘要
通过使用多重二体菌株，基因arg1和arg8分别被排除在除（i）第十五号染色体和（ii）第九号和第十五号染色体之外的所有第一号至第十七号染色体之外。进一步的非整倍体分析表明，这两个基因位于同一条染色体上。通过四分体分析，发现arg1与第十五号染色体左臂上的SUP3连锁（亲二型：非亲二型：四型 = 74；6：139），并且arg8与同一条臂上的arg1松散连锁（亲二型：非亲二型：四型 = 72：17：220）。该染色体臂上基因的顺序是着丝粒-SUP3-arg8。由于arg1先前已被用于定义第十八号染色体，这些结果重新确定了酿酒酵母中的最小染色体数为17条。
相似文献
1
Genetic mapping of arg1 and arg8 in Saccharomyces cerevisiae by trisomic analysis combined with interallelic complementation.通过三体分析结合等位基因间互补对酿酒酵母中arg1和arg8进行基因定位。
J Bacteriol. 1980 Jan;141(1):270-4. doi: 10.1128/jb.141.1.270-274.1980.
2
Genetic mapping of two pairs of linked ribosomal protein genes in Saccharomyces cerevisiae.酿酒酵母中两对连锁核糖体蛋白基因的遗传图谱
Curr Genet. 1987;11(6-7):445-50. doi: 10.1007/BF00384605.
3
Chromosomes XIV and XVII of Saccharomyces cerevisiae constitute a single linkage group.酿酒酵母的第十四号和十七号染色体构成一个单一连锁群。
Mol Cell Biol. 1982 Nov;2(11):1399-409. doi: 10.1128/mcb.2.11.1399-1409.1982.
4
Regulation of arginine biosynthesis in Saccharomyces cerevisiae: isolation of a cis-dominant, constitutive mutant for ornithine carbamoyltransferase synthesis.酿酒酵母中精氨酸生物合成的调控：鸟氨酸氨甲酰基转移酶合成的顺式显性组成型突变体的分离。
J Bacteriol. 1976 Oct;128(1):49-55. doi: 10.1128/jb.128.1.49-55.1976.
5
Genetic mapping of 1,3-beta-glucanase-encoding genes in Saccharomyces cerevisiae.酿酒酵母中1,3-β-葡聚糖酶编码基因的遗传定位
Curr Genet. 1992 Oct;22(4):283-8. doi: 10.1007/BF00317922.
6
Mapping of DBR1 and YPK1 suggests a major revision of the genetic map of the left arm of Saccharomyces cerevisiae Chromosome XI.DBR1和YPK1的定位表明酿酒酵母第十一号染色体左臂遗传图谱需进行重大修订。
Genetics. 1994 Oct;138(2):283-7. doi: 10.1093/genetics/138.2.283.
7
The PHO80/TUP7 locus in Saccharomyces cerevisiae is on the left arm of chromosome XV: mapping by chromosome engineering.酿酒酵母中的PHO80/TUP7基因座位于第十五号染色体的左臂：通过染色体工程进行定位。
Yeast. 1991 Nov;7(8):859-65. doi: 10.1002/yea.320070812.
8
Mapping of a centromere-linked gene responsible for constitutive acid phosphatase synthesis in yeast.酵母中负责组成型酸性磷酸酶合成的着丝粒连锁基因的定位
Mol Gen Genet. 1980;180(3):605-7. doi: 10.1007/BF00268067.
9
Evidence for a new chromosome in Saccharomyces cerevisiae.酿酒酵母中一条新染色体的证据。
Mol Cell Biol. 1983 Mar;3(3):415-20. doi: 10.1128/mcb.3.3.415-420.1983.
10
DSLINK: a computer program for gene-centromere linkage analysis in families with a trisomic offspring.DSLINK：一种用于对有三体后代的家庭进行基因-着丝粒连锁分析的计算机程序。
Am J Hum Genet. 1987 Sep;41(3):350-5.
引用本文的文献
1
Vitamin requirements and biosynthesis in Saccharomyces cerevisiae.酿酒酵母中的维生素需求与生物合成。
Yeast. 2020 Apr;37(4):283-304. doi: 10.1002/yea.3461. Epub 2020 Feb 6.
2
A novel mutation that affects utilization of galactose in Saccharomyces cerevisiae II. A partial aneuploid for chromosome II isolated as a revertant of the mutant.影响酿酒酵母 II 中半乳糖利用的一种新突变。作为突变体回复突变体分离出的 II 号染色体部分三体。
Curr Genet. 1981 May;3(2):91-6. doi: 10.1007/BF00365711.
3
Genetic mapping of arg, cpa, car and tsm genes in Saccharomyces cerevisiae by trisomic analysis.通过三体分析对酿酒酵母中的 arg、cpa、car 和 tsm 基因进行遗传定位。
Curr Genet. 1982 Nov;6(2):93-8. doi: 10.1007/BF00435206.
4
Regional bivalent-univalent pairing versus trivalent pairing of a trisomic chromosome in Saccharomyces cerevisiae.酿酒酵母中三体染色体的区域二价体-单价体配对与三价体配对
Genetics. 1996 Nov;144(3):957-66. doi: 10.1093/genetics/144.3.957.
5
SCM2, a tryptophan permease in Saccharomyces cerevisiae, is important for cell growth.SCM2是酿酒酵母中的一种色氨酸通透酶，对细胞生长很重要。
Mol Gen Genet. 1994 Aug 2;244(3):260-8. doi: 10.1007/BF00285453.
6
Differential mitotic stability of yeast disomes derived from triploid meiosis.源自三倍体减数分裂的酵母二体的有丝分裂稳定性差异
Genetics. 1981 Jun;98(2):239-55. doi: 10.1093/genetics/98.2.239.
7
Genetic map of Saccharomyces cerevisiae.酿酒酵母的遗传图谱。
Microbiol Rev. 1980 Dec;44(4):519-71. doi: 10.1128/mr.44.4.519-571.1980.
8
Chromosomes XIV and XVII of Saccharomyces cerevisiae constitute a single linkage group.酿酒酵母的第十四号和十七号染色体构成一个单一连锁群。
Mol Cell Biol. 1982 Nov;2(11):1399-409. doi: 10.1128/mcb.2.11.1399-1409.1982.
9
Genetic effects of methyl benzimidazole-2-yl-carbamate on Saccharomyces cerevisiae.甲基苯并咪唑-2-基氨基甲酸酯对酿酒酵母的遗传效应。
Mol Cell Biol. 1982 Sep;2(9):1064-79. doi: 10.1128/mcb.2.9.1064-1079.1982.
10
Evidence for a new chromosome in Saccharomyces cerevisiae.酿酒酵母中一条新染色体的证据。
Mol Cell Biol. 1983 Mar;3(3):415-20. doi: 10.1128/mcb.3.3.415-420.1983.
本文引用的文献
1
Genetic Mapping in Saccharomyces IV. Mapping of Temperature-Sensitive Genes and Use of Disomic Strains in Localizing Genes.酵母的遗传图谱 IV. 温度敏感基因的图谱绘制以及在定位基因中的二倍体菌株的使用。
Genetics. 1973 May;74(1):33-54. doi: 10.1093/genetics/74.1.33.
2
Chromosome Mapping in Saccharomyces: Centromere-Linked Genes.酿酒酵母中的染色体图谱：着丝粒连锁基因。
Genetics. 1960 Aug;45(8):1085-110. doi: 10.1093/genetics/45.8.1085.
3
Biochemical Mutants in the Smut Fungus Ustilago Maydis.玉米黑粉菌中的生化突变体
Genetics. 1949 Sep;34(5):607-26. doi: 10.1093/genetics/34.5.607.
4
Genetic mapping in Saccharomyces.酿酒酵母中的基因图谱
Genetics. 1966 Jan;53(1):165-73. doi: 10.1093/genetics/53.1.165.
5
Genetic mapping of nonsense suppressors in yeast.酵母中无义抑制基因的遗传图谱
Genetics. 1968 Dec;60(4):735-42. doi: 10.1093/genetics/60.4.735.
6
The tolerance of aneuploidy in yeast.酵母中非整倍体的耐受性。
Genet Res. 1970 Dec;16(3):333-40. doi: 10.1017/s0016672300002597.
7
Genetic mapping in yeast.酵母中的遗传图谱
Methods Cell Biol. 1975;11:221-33. doi: 10.1016/s0091-679x(08)60325-8.
8
Isolation and characterization of Saccharomyces cerevisiae mutants deficient in S-adenosylmethionine decarboxylase, spermidine, and spermine.缺乏S-腺苷甲硫氨酸脱羧酶、亚精胺和精胺的酿酒酵母突变体的分离与鉴定
J Bacteriol. 1978 Apr;134(1):208-13. doi: 10.1128/jb.134.1.208-213.1978.
9
Yeast argininosuccinate synthetase. Purification; structural and kinetic properties.
Eur J Biochem. 1979 Feb 15;94(1):153-63. doi: 10.1111/j.1432-1033.1979.tb12882.x.
Zotero 插件