通过三体分析结合等位基因间互补对酿酒酵母中arg1和arg8进行基因定位。
Genetic mapping of arg1 and arg8 in Saccharomyces cerevisiae by trisomic analysis combined with interallelic complementation.
作者信息
Hilger F, Mortimer R K
出版信息
J Bacteriol. 1980 Jan;141(1):270-4. doi: 10.1128/jb.141.1.270-274.1980.
Abstract
Through use of multiply disomic strains, the genes arg1 and arg8 were excluded from all of chromosomes I to XVII except (i) XV and (ii) IX and XV, respectively. Further aneuploid analyses showed that these two genes were on the same chromosome. By tetrad analysis, arg1 was shown to be linked to SUP3 on the left arm of chromosome XV (parental ditype:nonparental ditype:tetratype = 74; 6:139) and arg8 was shown to be loosely linked to arg1 (parental ditype:nonparental ditype:tetratype 72:17:220) on the same arm. The sequence of the genes on this chromosome arm is centromere-SUP3-arg8. Because arg1 had previously been used to define an 18th chromosome, these results reestablished the minimum chromosome number in Saccharomyces cerevisiae as 17.
摘要
通过使用多重二体菌株，基因arg1和arg8分别被排除在除（i）第十五号染色体和（ii）第九号和第十五号染色体之外的所有第一号至第十七号染色体之外。进一步的非整倍体分析表明，这两个基因位于同一条染色体上。通过四分体分析，发现arg1与第十五号染色体左臂上的SUP3连锁（亲二型：非亲二型：四型 = 74；6：139），并且arg8与同一条臂上的arg1松散连锁（亲二型：非亲二型：四型 = 72：17：220）。该染色体臂上基因的顺序是着丝粒-SUP3-arg8。由于arg1先前已被用于定义第十八号染色体，这些结果重新确定了酿酒酵母中的最小染色体数为17条。
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