Department of Biochemistry (U38-FCT), Faculty of Medicine, University of Porto , 4200-319 Porto, Portugal.
J Nat Prod. 2013 Nov 22;76(11):2047-53. doi: 10.1021/np4002898. Epub 2013 Nov 7.
Type 1 diabetes mellitus is responsible for metabolic dysfunction, accompanied by chronic inflammation, oxidative stress, and endothelium dysfunction, and is often associated with impaired wound healing. Phenol-rich food improves vascular function, contributing to diabetes prevention. This study has evaluated the effect of phenol-rich beverage consumption in diabetic rats on wound healing, through angiogenesis, inflammation, and oxidative stress modulation. A wound-healing assay was performed in streptozotocin-induced diabetic Wistar rats drinking water, 5% ethanol, and stout beer with and without 10 mg/L xanthohumol (1), for a five-week period. Wounded skin microvessel density was reduced to normal values upon consumption of 1 in diabetic rats, being accompanied by decreased serum VEGF-A and inflammatory markers (IL-1β, NO, N-acetylglucosaminidase). Systemic glutathione and kidney and liver H2O2, 3-nitrotyrosine, and protein carbonylation also decreased to healthy levels after treatment with 1, implying an improvement in oxidative stress status. These findings suggest that consumption of xanthohumol (1) by diabetic animals consistently decreases inflammation and oxidative stress, allowing neovascularization control and improving diabetic wound healing.
1 型糖尿病可导致代谢功能障碍，伴有慢性炎症、氧化应激和血管内皮功能障碍，常伴有伤口愈合受损。富含酚类的食物可改善血管功能，有助于预防糖尿病。本研究评估了富含酚类饮料对饮用含或不含 10 mg/L 黄腐酚（1）水、5%乙醇和黑啤酒的链脲佐菌素诱导的糖尿病 Wistar 大鼠伤口愈合的影响，为期五周。在糖尿病大鼠中，摄入 1 可将皮肤微血管密度恢复至正常水平，同时降低血清 VEGF-A 和炎症标志物（IL-1β、NO、N-乙酰氨基葡萄糖苷酶）。在使用 1 处理后，全身谷胱甘肽以及肾脏和肝脏的 H2O2、3-硝基酪氨酸和蛋白质羰基化也降低至健康水平，表明氧化应激状态得到改善。这些发现表明，糖尿病动物摄入黄腐酚（1）可持续降低炎症和氧化应激，从而控制新血管生成并改善糖尿病伤口愈合。
