Asbrink E, Hovmark A, Hederstedt B
Acta Derm Venereol. 1985;65(6):509-14.
To determine whether antibodies to Borrelia spirochetes were present, sera from 88 patients with uncomplicated erythema chronicum migrans Afzelius (ECMA), from 9 patients with ECMA-related extracutaneous complications and from 26 patients with acrodermatitis chronica atrophicans (ACA) were submitted to an enzyme-linked immunosorbent assay (ELISA) and an indirect immunofluorescence (IF) assay. The assays were calculated to be 95% specific. There was good correlation between the IF test with a polyvalent conjugate and IgG ELISA. Of patients with uncomplicated ECMA, 18% were seropositive by IgG ELISA and 11% by IgM ELISA, and 15% showed elevated IF titers. Elevated serum antibody levels of IgG as measured by ELISA and elevated IF titers were found in all patients with extracutaneous complications and in the patients with ACA. Declining IgG titers were observed at follow-up 6-12 months after therapy, but the majority of the patients with ACA were still seropositive.
为确定是否存在抗疏螺旋体抗体，对88例单纯性慢性游走性红斑阿费利乌斯型（ECMA）患者、9例伴有ECMA相关皮肤外并发症的患者以及26例慢性萎缩性肢端皮炎（ACA）患者的血清进行了酶联免疫吸附测定（ELISA）和间接免疫荧光（IF）测定。这些测定的特异性经计算为95%。使用多价结合物的IF试验与IgG ELISA之间存在良好相关性。在单纯性ECMA患者中，IgG ELISA血清阳性率为18%，IgM ELISA血清阳性率为11%，15%的患者IF滴度升高。在所有伴有皮肤外并发症的患者以及ACA患者中均发现通过ELISA测定的IgG血清抗体水平升高以及IF滴度升高。在治疗后6 - 12个月的随访中观察到IgG滴度下降，但大多数ACA患者仍为血清阳性。
