National Research Council of Canada, Plant Biotechnology Institute, 110 Gymnasium Road, S7N 0W9, Saskatoon, Saskatchewan, Canada.
Plant Cell Rep. 1993 Jan;12(2):89-94. doi: 10.1007/BF00241941.
Spring wheat (Triticum aestivum L.) zygotic embryos were successfully cryopreserved, without the addition of exogenous cryoprotectants, using only an abscisic acid (ABA) pretreatment. Optimum survival was obtained when embryos were cultured in vitro for 10 days on semisolid Murashige and Skoog (MS) nutrient medium supplemented with 0.5 mg/L (±) ABA prior to cryopreservation. The embryos resumed growth within three days when returned to MS medium devoid of ABA but containing 2mg/L 2,4-dichlorophenoxyacetic acid. The embryogenic calli produced from these embryos exhibited normal plant regeneration on auxin-free media. Changes in dw/fw ratio, as well as the esterified fatty acid and sucrose concentrations correlated positively with the development of tolerance to cryopreservation.
春小麦（Triticum aestivum L.）合子胚经 ABA 预处理，无需添加外源冷冻保护剂，成功冷冻保存。当胚在补充有 0.5mg/L(±)ABA 的半固态 Murashige 和 Skoog（MS）营养培养基中体外培养 10 天后再进行冷冻保存时，可获得最佳存活率。当将胚返回不含 ABA 但含有 2mg/L 2,4-二氯苯氧乙酸的 MS 培养基中时，胚在三天内恢复生长。从这些胚中产生的胚性愈伤组织在不含生长素的培养基上表现出正常的植株再生。dw/fw 比值、酯化脂肪酸和蔗糖浓度的变化与对冷冻保存的耐受性的发展呈正相关。
