Wulff K
Arzneimittelforschung. 1986;36(1):157-61.
The method of nucleic acid hybridization opens up new possibilities in laboratory diagnosis. This method, which enables us to assay for definite nucleic acid sequences in the specimen, has the advantage of high specificity and a strong binding force between the analyte nucleic acid and the nucleic acid probe. So far this method primarily works with radioactive labels. The development of non-radioactive detection systems suited for routine laboratories with sufficient sensitivity is still at its origin. One broad field of application for nucleic acid hybridization is the detection of the genome of certain viruses or bacteria in the specimen. Another more future oriented potential of these methods lies in the investigation and diagnosis of inborn errors of metabolism.
核酸杂交方法为实验室诊断开辟了新的可能性。这种方法能够测定标本中特定的核酸序列，具有特异性高以及分析物核酸与核酸探针之间结合力强的优点。到目前为止，该方法主要使用放射性标记。适用于常规实验室且具有足够灵敏度的非放射性检测系统仍处于起步阶段。核酸杂交的一个广泛应用领域是检测标本中某些病毒或细菌的基因组。这些方法另一个更具前瞻性的潜在应用在于先天性代谢缺陷的研究和诊断。
