Antifungal activities of quince seed mucilage hydrogel decorated with essential oils of and .

BACKGROUND AND OBJECTIVES

Candidiasis and pityriasis versicolor are opportunistic fungal infections that are caused by spp. and spp. yeasts. Conventional drugs like azole and amino derivatives are known to treat fungal skin diseases. However, drawbacks like long-term side effects and drug resistance lead to investigate on antifungal properties of phytochemicals as an alternative to available synthetic drugs.

MATERIALS AND METHODS

The herbal nano hydrogel was successfully synthesized from Quince Seed extract followed by ultrasonic treatment and it has been formulated using a mixture of essential oils. We evaluated the antifungal in vitro assay for a mixture of essential oils in combination with herbal nano hydrogel against and strains by micro dilution method.

RESULTS

The results indicated that essential oils possess antifungal activity with the MIC value of 12.5 and 6.24 mg/ml against and , respectively. No fungicidal effect was reported for the herbal hydrogel before nanofabrication while it shown some antifungal activity after ultrasonic treatment for 5 and 10 minutes. As anticipated; the antifungal property of essential oil mixture was appreciably improved when it combined with herbal nano hydrogel where the highest level of inhibition was observed at concentration of 3.125 mg/ml for both strains. The loss in biological activity observed when the ultrasonic treatment on herbal nano hydrogel performed for longer time.

CONCLUSION

The proposed plant-based nano formulation shown promising in vitro antifungal activities against and strains and its antifungal properties were comparable with commercially available agents like clotrimazole. The new formulation expected to be safe with minimum long-term side effects. Further investigations are underway to confirm the safety and the mechanism of the action of this new herbal formulation.