John Innes Institute, Colney Lane, NR4 7UH, Norwich, UK.
Theor Appl Genet. 1991 Jun;81(6):800-5. doi: 10.1007/BF00224993.
The copy number, genomic arrangement and linkage relationships of two classes of lipoxygenase gene have been investigated in Pisum(pea) lines. Each of the two classes contained two to three members in P. sativum lines. RFLPs associated with genomic fragments containing the 5' sequences of one gene class permitted its correlation in genetical analyses with a lipoxygenase locus on linkage group 4, which was previously identified through polypeptide variation. Genetical analyses of RFLPs associated with other fragments identified by low- and medium-stringency hybridization to lipoxygenase cDNAs indicate the existence of other unlinked lipoxygenase gene loci.
已经研究了豌豆(Pisum)品系中两类脂氧合酶基因的拷贝数、基因组排列和连锁关系。在 P. sativum 品系中,每类基因包含两个到三个成员。与包含一类基因 5'序列的基因组片段相关的 RFLP 允许其在遗传分析中与先前通过多肽变化鉴定的连锁群 4 上的脂氧合酶位点相关联。与脂氧合酶 cDNA 的低和中严格杂交鉴定的其他片段相关的 RFLP 的遗传分析表明存在其他不连锁的脂氧合酶基因座。