Rodríguez-Concepción M, Beltrán J P
Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-CSIC, Spain.
Plant Mol Biol. 1995 Mar;27(5):887-99. doi: 10.1007/BF00037017.
A cDNA clone (loxg) corresponding to a gene repressed during carpel development has been isolated from a cDNA library of unpollinated carpels induced to grow by treatment with gibberellic acid (GA3). The sequences of loxg cDNA and the deduced polypeptide have a high similarity with legume type 2 lipoxygenases, especially with Phaseolus lox1 (78.5% similarity at the protein level) and pea and soybean lox3 (83.6% and 85.4%, respectively). loxg expression is constant in unstimulated carpels but it decreases in carpels induced to keep growing by fertilization or hormone treatment. A similar pattern of repression was observed in lipoxygenase activity of pea and tomato carpels. In situ hybridization studies showed that loxg mRNAs are present in the endocarp and the mesocarp of pea pods; no loxg expression was detectable either in the pod exocarp or in the ovules. Loxg is also expressed in other young growing tissues, especially in flower organs. Nevertheless, the natural pattern of flower and fruit development is associated with loxg repression.
通过用赤霉素（GA3）处理诱导未授粉心皮生长，从其cDNA文库中分离出了一个与心皮发育过程中被抑制的基因相对应的cDNA克隆（loxg）。loxg cDNA序列和推导的多肽与豆科植物2型脂氧合酶具有高度相似性，尤其是与菜豆lox1（蛋白质水平相似度为78.5%）以及豌豆和大豆lox3（分别为83.6%和85.4%）。loxg在未受刺激的心皮中表达恒定，但在通过受精或激素处理诱导持续生长的心皮中表达下降。在豌豆和番茄心皮的脂氧合酶活性中也观察到了类似的抑制模式。原位杂交研究表明，loxg mRNA存在于豌豆荚的内果皮和中果皮中；在荚的外果皮或胚珠中均未检测到loxg表达。Loxg也在其他幼嫩生长组织中表达，尤其是在花器官中。然而，花和果实发育的自然模式与loxg的抑制相关。
