Nicolau Korres Adriana Marcia, Aquije Gloria Maria de Farias V, Buss David S, Ventura Jose Aires, Fernandes Patricia Machado Bueno, Fernandes Antonio Alberto Ribeiro
Núcleo de Biotecnologia, Universidade Federal do Espírito Santo, Avenida Marechal Campos 1468, 29040-090 Vitória, ES, Brazil ; Instituto Federal de Educação, Ciência e Tecnologia do Espírito Santo, Campus Vitória, Avenida Vitória 1729, 29040-780 Vitória, ES, Brazil.
ScientificWorldJournal. 2013 Oct 10;2013:925375. doi: 10.1155/2013/925375. eCollection 2013.
Some bacterial species can colonize humans and plants. It is almost impossible to prevent the contact of clinically pathogenic bacteria with food crops, and if they can persist there, they can reenter the human food chain and cause disease. On the leaf surface, microorganisms are exposed to a number of stress factors. It is unclear how they survive in such different environments. By increasing adhesion to diverse substrates, minimizing environmental differences, and providing protection against defence mechanisms, biofilms could provide part of the answer. Klebsiella pneumoniae subsp. pneumoniae is clinically important and also associated with fruit diseases, such as "pineapple fruit collapse." We aimed to characterize biofilm formation and adhesion mechanisms of this species isolated from pineapple in comparison with a clinical isolate. No differences were found between the two isolates quantitatively or qualitatively. Both tested positive for capsule formation and were hydrophobic, but neither produced adherence fibres, which might account for their relatively weak adhesion compared to the positive control Staphylococcus epidermidis ATCC 35984. Both produced biofilms on glass and polystyrene, more consistently at 40°C than 35°C, confirmed by atomic force and high-vacuum scanning electron microscopy. Biofilm formation was maintained in an acidic environment, which may be relevant phytopathologically.
一些细菌物种能够在人类和植物上定殖。几乎不可能防止临床致病细菌与粮食作物接触，而且如果它们能在作物上存活下来，就可能重新进入人类食物链并引发疾病。在叶片表面，微生物会面临多种应激因素。目前尚不清楚它们如何在如此不同的环境中存活。生物膜通过增强对多种底物的黏附、最小化环境差异以及提供针对防御机制的保护作用，可能会给出部分答案。肺炎克雷伯菌肺炎亚种在临床上很重要，并且还与水果疾病有关，例如“菠萝果实萎蔫病”。我们旨在比较从菠萝中分离出的该菌种与临床分离株的生物膜形成和黏附机制。在定量或定性方面，未发现这两种分离株之间存在差异。两者的荚膜形成检测均呈阳性且具有疏水性，但均未产生黏附纤维，这可能解释了它们与阳性对照表皮葡萄球菌ATCC 35984相比黏附性相对较弱的原因。两者在玻璃和聚苯乙烯上均能形成生物膜，在40°C时比35°C时更稳定，这通过原子力显微镜和高真空扫描电子显微镜得以证实。生物膜的形成在酸性环境中得以维持，这在植物病理学上可能具有相关性。
