Munukka Eveliina, Leppäranta Outi, Korkeamäki Mika, Vaahtio Minna, Peltola Timo, Zhang Di, Hupa Leena, Ylänen Heimo, Salonen Jukka I, Viljanen Matti K, Eerola Erkki
Department of Medical Microbiology, University of Turku, Kiinamyllynkatu 13, Turku 20500, Finland.
J Mater Sci Mater Med. 2008 Jan;19(1):27-32. doi: 10.1007/s10856-007-3143-1. Epub 2007 Jun 14.
Bioactive glasses (BAGs) have been studied for decades for clinical use, and they have found many dental and orthopedic applications. BAGs have also been shown to have an antibacterial effect e.g., on some oral microorganisms. In this extensive work we show that six powdered BAGs and two sol-gel derived materials have a clear antibacterial effect on 29 clinically important bacterial species. We also incorporated a rapid and accurate flow cytometric (FCM) method to calculate and standardize the numbers of viable bacteria inoculated in the suspensions used in the tests for antibacterial activity. In all materials tested growth inhibition could be demonstrated, although the concentration and time needed for the effect varied depending on the BAG. The most effective glass was S53P4, which had a clear growth-inhibitory effect on all pathogens tested. The sol-gel derived materials CaPSiO and CaPSiO II also showed a strong antibacterial effect. In summary, BAGs were found to clearly inhibit the growth of a wide selection of bacterial species causing e.g., infections on the surfaces of prostheses in the body after implantation.
生物活性玻璃（BAGs）已被研究数十年用于临床，并且已在许多牙科和骨科领域得到应用。BAGs还被证明具有抗菌作用，例如对某些口腔微生物。在这项广泛的研究中，我们表明六种粉末状BAGs和两种溶胶-凝胶衍生材料对29种临床重要细菌具有明显的抗菌作用。我们还采用了一种快速准确的流式细胞术（FCM）方法来计算和标准化用于抗菌活性测试的悬浮液中接种的活细菌数量。在所有测试材料中均能证明生长抑制作用，尽管产生这种效果所需的浓度和时间因BAG而异。最有效的玻璃是S53P4，它对所有测试的病原体都有明显的生长抑制作用。溶胶-凝胶衍生材料CaPSiO和CaPSiO II也显示出很强的抗菌作用。总之，发现BAGs能明显抑制多种细菌的生长，这些细菌会导致例如植入后体内假体表面的感染。
