Research Group of Bacterial Genomics and Antimicrobial Resistance, National Food Institute,Technical University of Denmark,Lyngby,Denmark.
Research Group of Epidemiology and Risk Modelling, National Food Institute,Technical University of Denmark,Søborg,Denmark.
Epidemiol Infect. 2014 Aug;142(8):1727-36. doi: 10.1017/S095026881300280X. Epub 2013 Nov 13.
To identify a cost-effective and practical method for detection of methicillin-resistant Staphylococcus aureus (MRSA) in pig herds, the relative sensitivity of four sample types: nasal swabs, ear-skin (skin behind the ears) swabs, environmental dust swabs and air was compared. Moreover, dependency of sensitivity on within-herd prevalence was estimated. spa-typing was applied in order to study strain diversity. The sensitivity of one air sample was equal to the sensitivity of ten pools of five nasal swabs and relatively independent of within-herd prevalence [predicted to be nearly perfect (99%) for within-herd prevalence ⩾25%]. The results indicate that taking swabs of skin behind the ears (ten pools of five) was even more sensitive than taking nasal swabs (ten pools of five) at the herd level and detected significantly more positive samples. spa types t011, t034 and t4208 were observed. In conclusion, MRSA detection by air sampling is easy to perform, reduces costs and analytical time compared to existing methods, and is recommended for initial testing of herds. Ear-skin swab sampling may be more sensitive for MRSA detection than air sampling or nasal swab sampling.
为了确定一种经济实用的方法来检测猪群中的耐甲氧西林金黄色葡萄球菌（MRSA），我们比较了四种样本类型（鼻拭子、耳后皮肤拭子、环境灰尘拭子和空气）的相对敏感性。此外，还估计了敏感性对群体内流行率的依赖性。通过 spa 分型来研究菌株多样性。一份空气样本的敏感性与十份五份鼻拭子的敏感性相同，且与群体内流行率相对独立[群体内流行率≥25%时，预测为接近完美（99%）]。结果表明，在群体水平上，采集耳后皮肤拭子（十份五份）比采集鼻拭子（十份五份）更为敏感，并且检测到更多的阳性样本。观察到 spa 类型 t011、t034 和 t4208。总之，与现有方法相比，空气采样法检测 MRSA 既易于操作，又降低了成本和分析时间，推荐用于群体的初步检测。与空气采样或鼻拭子采样相比，耳后皮肤拭子采样可能对 MRSA 检测更敏感。
