Tumor Immunology Laboratory, IdiPAZ, La Paz Hospital, Spain.
Biochem Biophys Res Commun. 2013 Nov 29;441(4):947-52. doi: 10.1016/j.bbrc.2013.11.012. Epub 2013 Nov 14.
Although the natural mode of bacterial growth in nature is as biofilm, almost all antimicrobial and immunological tests are routinely developed using planktonic inoculums. Bacterial biofilms protect the microbial community from external damage and promote the persistence of chronic infections. In this study, interactions between human macrophages and bacterial inoculums of planktonic and biofilm modes of growth have been explored using Escherichia coli (E. coli) K12. Human macrophages phagocytize planktonic E. coli more efficiently than bacteria grown in a biofilm. Moreover, they prefer to phagocytize planktonic bacteria. In this context, CD64 expression is involved. Our data indicate that bacteria with "a biofilm background" avoid phagocytosis by naïve macrophages, which could create a favorable environment for chronic infection. Our findings were corroborated in a clinical O25b-ST131 ESBL-producer E. coli isolate, which caused urinary tract infections.
虽然细菌在自然界中的自然生长模式是生物膜，但几乎所有的抗菌和免疫测试通常都是使用浮游生物接种物来开发的。细菌生物膜保护微生物群落免受外部伤害，并促进慢性感染的持续存在。在这项研究中，使用大肠杆菌（E. coli）K12 探索了人类巨噬细胞与浮游生物和生物膜生长模式的细菌接种物之间的相互作用。人类巨噬细胞吞噬浮游大肠杆菌的效率高于生物膜中生长的细菌。此外，它们更喜欢吞噬浮游细菌。在这种情况下，CD64 表达参与其中。我们的数据表明，具有“生物膜背景”的细菌可以避免原始巨噬细胞的吞噬作用，这可能为慢性感染创造有利环境。我们的发现在引起尿路感染的临床 O25b-ST131 ESBL 产肠杆菌分离株中得到了证实。
