Dipartimento di Biologia Cellulare, Università di Camerino, Via F. Camerini 2, I-62032, Camerino, Italy.
Theor Appl Genet. 1985 Dec;71(2):330-3. doi: 10.1007/BF00252076.
The analysis of cress seeds from Thebes dated approximately 1400 years B.C. showed that fragments of RNA up to 10 bases in length were still present in the ancient seeds. After having been made radioactive at the 5'OH terminus, the RNA fragments were used as probes in a spot hybridization experiment. They were shown to hybridize to cress DNA and, to a lesser extent, to that of phylogenetically distant species. When fixed onto nitrocellulose and probed with different cloned genes, the RNA fragments were shown to originate from breakage of the 25 and 18s cytoplasmic rRNA.
对来自底比斯的大约公元前 1400 年的水芹种子进行分析后发现，在这些古老的种子中仍存在长度达 10 个碱基的 RNA 片段。在 5'OH 末端被放射性标记后，这些 RNA 片段被用作斑点杂交实验的探针。实验表明，它们与水芹 DNA 杂交，并且在较小程度上与进化上相距较远的物种的 DNA 杂交。当将这些 RNA 片段固定在硝酸纤维素上并用不同的克隆基因进行探测时，表明它们源自 25s 和 18s 细胞质 rRNA 的断裂。
