CERM and Department of Chemistry "Ugo Schiff", Via L. Sacconi 6, 50019, Sesto Fiorentino (Fi), Italy.
Chem Commun (Camb). 2014 Jan 14;50(4):421-3. doi: 10.1039/c3cc46896h.
Bioinspired silica coprecipitates of enzymes find a wide range of applications for analysis and catalysis in industrial and academic research. Here we used SSNMR as a proxy for the 3D structure of enzymes trapped in bioinspired silica. We show that it is easy to assess whether the enzymes retain their native conformation in atomic detail. We thus propose SSNMR as a rapid and reliable tool to analyze this kind of samples at high resolution.
受生物启发的酶硅共沉淀物在工业和学术研究的分析和催化中有着广泛的应用。在这里，我们使用固态 NMR（SSNMR）作为酶在受生物启发的硅中捕获的 3D 结构的替代物。我们表明，很容易评估酶是否以原子细节保留其天然构象。因此，我们提出 SSNMR 作为一种快速可靠的工具，可用于高分辨率分析此类样品。
