Quinn Caitlin M, Wang Mingzhang, Polenova Tatyana
Department of Chemistry and Biochemistry, University of Delaware, 036 Brown Laboratories, Newark, DE, 19716, USA.
Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, 1051 Biomedical Science Tower 3, 3501 Fifth Ave, Pittsburgh, PA, 15261, USA.
Methods Mol Biol. 2018;1688:1-35. doi: 10.1007/978-1-4939-7386-6_1.
As a result of profound gains in sensitivity and resolution afforded by ultrahigh magnetic fields, transformative applications in the fields of structural biology and materials science are being realized. The development of dual low temperature superconducting (LTS)/high-temperature superconducting (HTS) magnets has enabled the achievement of magnetic fields above 1 GHz (23.5 T), which will open doors to an unprecedented new range of applications. In this contribution, we discuss the promise of ultrahigh field magnetic resonance. We highlight several methodological developments pertinent at high-magnetic fields including measurement of H-H distances and H chemical shift anisotropy in the solid state as well as studies of quadrupolar nuclei such as O. Higher magnetic fields have advanced heteronuclear detection in solution NMR, valuable for applications including metabolomics and disordered proteins, as well as expanded use of proton detection in the solid state in conjunction with ultrafast magic angle spinning. We also present several recent applications to structural studies of the AP205 bacteriophage, the M2 channel from Influenza A, and biomaterials such as human bone. Gains in sensitivity and resolution from increased field strengths will enable advanced applications of NMR spectroscopy including in vivo studies of whole cells and intact virions.
由于超高磁场带来的灵敏度和分辨率的显著提升，结构生物学和材料科学领域正在实现变革性应用。双低温超导（LTS）/高温超导（HTS）磁体的发展使得能够实现超过1 GHz（23.5 T）的磁场，这将为一系列前所未有的新应用打开大门。在本文中，我们讨论了超高场磁共振的前景。我们强调了在高磁场下相关的几个方法学进展，包括固态中H-H距离和H化学位移各向异性的测量以及诸如O等四极核的研究。更高的磁场推动了溶液核磁共振中的异核检测，这对于包括代谢组学和无序蛋白质在内的应用很有价值，同时也扩大了固态中质子检测与超快魔角旋转相结合的应用。我们还介绍了最近在AP205噬菌体、甲型流感病毒M2通道以及人体骨骼等生物材料结构研究中的几个应用。场强增加带来的灵敏度和分辨率的提升将使核磁共振光谱能够进行高级应用，包括对全细胞和完整病毒粒子的体内研究。
