Gigli Lucia, Ravera Enrico, Calderone Vito, Luchinat Claudio
Magnetic Resonance Center (CERM), University of Florence, and Consorzio Interuniversitario Risonanze Magnetiche di Metalloproteine (CIRMMP), 50019 Sesto Fiorentino, Italy.
Department of Chemistry "Ugo Schiff", University of Florence, 50019 Sesto Fiorentino, Italy.
Biomolecules. 2020 Dec 30;11(1):43. doi: 10.3390/biom11010043.
Nature has evolved several molecular machineries to promote the formation at physiological conditions of inorganic materials, which would otherwise be formed in extreme conditions. The molecular determinants of this process have been established over the last decade, identifying a strong role of electrostatics in the first steps of the precipitation. However, no conclusive, structure-based evidence has been provided so far. In this manuscript, we test the binding of lysozyme with silica and titania potential precursors. In contrast with the absence of structural information about the interaction with the silica precursor, we observe the interaction with a mononuclear titanium(IV) species, which is found to occur in a region rich of positive charges.
自然界已经进化出了几种分子机制，以促进在生理条件下形成无机材料，否则这些无机材料将在极端条件下形成。在过去十年中已经确定了这一过程的分子决定因素，发现静电在沉淀的第一步中起着重要作用。然而，迄今为止尚未提供确凿的、基于结构的证据。在本论文中，我们测试了溶菌酶与二氧化硅和二氧化钛潜在前体的结合。与缺乏关于与二氧化硅前体相互作用的结构信息相反，我们观察到与单核钛（IV）物种的相互作用，该物种存在于富含正电荷的区域。
