来自香叶天竺葵的EPs7630(®)可能通过DAF-16/FOXO信号通路增强秀丽隐杆线虫的抗应激能力。
EPs7630(®) from Pelargonium sidoides increases stress resistance in Caenorhabditis elegans probably via the DAF-16/FOXO pathway.
作者信息
Rezaizadehnajafi Leila, Wink Michael
机构信息
Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, INF 364, 69120 Heidelberg, Germany.
Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, INF 364, 69120 Heidelberg, Germany.
出版信息
Phytomedicine. 2014 Mar 15;21(4):547-50. doi: 10.1016/j.phymed.2013.10.027. Epub 2013 Nov 16.
EPs7630(®) a water alcohol extract of the roots from Pelargonium sidoides contains several secondary metabolites including highly oxygenated coumarins, various phenolics and polyphenols. Using the DPPH assay to measure antioxidant activity a free radical scavenging activity of 14.7±0.85μg/ml (IC50) was determined. As an in vivo model Caenorhabditis elegans was applied to study the effect of EPs7630(®) on stress resistance. EPs7630(®) treatment reduces intracellular hsp-16.2::GFP expression (induced by the pro-oxidant juglone) indicating that the secondary metabolites of EPs7630(®) are bioavailable and exhibit antioxidant activities in vivo. Application of EPs7630(®) (50μg/ml) to the transgenic mutant TJ356 induced the migration of the transcription factor DAF-16 from cytosol to the nucleus, suggesting a prominent role of DAF-16/FOXO in the daf-2 pathway for stress resistance.
EPs7630（®）是一种来自香叶天竺葵根的水醇提取物，含有多种次生代谢产物，包括高度氧化的香豆素、各种酚类和多酚类。使用DPPH法测定抗氧化活性，确定其自由基清除活性为14.7±0.85μg/ml（IC50）。作为体内模型，应用秀丽隐杆线虫来研究EPs7630（®）对抗逆性的影响。EPs7630（®）处理降低了细胞内热休克蛋白-16.2::GFP的表达（由促氧化剂胡桃醌诱导），表明EPs7630（®）的次生代谢产物具有生物利用度，并在体内表现出抗氧化活性。将EPs7630（®）（50μg/ml）应用于转基因突变体TJ356可诱导转录因子DAF-16从细胞质迁移到细胞核，表明DAF-16/FOXO在daf-2抗逆途径中起重要作用。
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