Department of Veterinary Pathology, Key Laboratory of Zoonosis of Ministry of Agriculture, China Agricultural University, Beijing 100193, China.
Virol J. 2013 Nov 19;10:341. doi: 10.1186/1743-422X-10-341.
Using various methods, we analyzed the cause of death among weaned pigs from a pig farm in Hebei Province, China. All 300 piglets (100% fatality) were identified as moribund, with death occurring within 1 month from the onset of clinical signs.
A single case exhibited obvious hemorrhagic necrotic changes with massive lymphocytic infiltration in multiple organs, in particular the liver, lungs and intestines. Dysplasia and lymphocyte deterioration were common in lymphatic organs. No visible bacterial colonies from liver and spleen were observed in nutrient, MacConkey, and blood agar plates. Using polymerase chain reaction techniques for this case, we attempted to detect a number of epidemic swine viruses in spleen and liver, including PRRSV, CSF, HEV, and PCV2. We found that this sample was positive for the presence of HEV and PRRSV.
We have detected HEV and PRRSV co-infection in one piglet. Severe pathologic changes were observed. The high mortality of weaned pigs which showed the similar clinical syptom was possibly a result of HEV and PRRSV co-infection, which has rarely been reported previously. We speculated that co-infection with PRRSV and HEV might lead to more serious problems.
我们使用多种方法分析了中国河北省一家猪场断奶仔猪的死亡原因。所有 300 头仔猪（死亡率为 100%）均被认定为病危，从出现临床症状到死亡不到 1 个月。
单个病例表现出明显的出血性坏死变化，多个器官（特别是肝脏、肺和肠道）有大量淋巴细胞浸润。淋巴器官中常见发育不良和淋巴细胞恶化。在营养琼脂、麦康凯琼脂和血琼脂平板上，未观察到肝脏和脾脏的可见细菌菌落。使用聚合酶链反应技术对该病例进行检测，我们试图检测猪繁殖与呼吸综合征病毒（PRRSV）、猪圆环病毒 2 型（PCV2）、猪传染性胃肠炎病毒（TGEV）和猪戊型肝炎病毒（HEV）等几种流行猪病病毒。我们发现该样本对 HEV 和 PRRSV 呈阳性。
我们在一只仔猪中检测到 HEV 和 PRRSV 混合感染。观察到严重的病理变化。表现出类似临床症状的断奶仔猪高死亡率可能是 HEV 和 PRRSV 混合感染的结果，以前很少有报道。我们推测 PRRSV 和 HEV 的混合感染可能导致更严重的问题。
