在中国污水处理厂的污水中检测到戊型肝炎病毒4型序列。
Hepatitis E Virus Genotype 4 Sequences Detected in Sewage from Treatment Plants of China.
作者信息
Li Heng, Li Wei, She Ruiping, Yu Liang, Wu Qiaoxing, Yang Jingling, Hu Fengjiao, Soomro Majid Hussain, Shi Ruihan, Hao Wenzhuo, Zhao Yue, Mao Jingjing
机构信息
Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Yuanmingyuan WestRoad 2#, Haidian District, Beijing, 100193, China.
Laboratory of Animal Pathology & Public Health, Key Laboratory of Zoonosis of the Ministry of Agriculture College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
出版信息
Food Environ Virol. 2017 Jun;9(2):230-233. doi: 10.1007/s12560-016-9276-y. Epub 2017 Jan 21.
The aim of this study was to investigate the occurrence of hepatitis E virus (HEV) in sewage samples in Shen Zhen, China. Sewage samples were collected from 152 sewage plants including livestock sewage, domestic sewage and treated sewage from May to July of 2015. Two of 152 samples were HEV positive (1.32%) from the livestock sewage plants. Partial ORF2 fragments of HEV were sequenced and a phylogenetic tree was constructed using MEGA5.1. Blast and phylogenetic analyses showed that both of these two sequences belonged to HEV Genotype 4. To the best of our knowledge, this is the first study on the molecular characterization of HEV in wastewater in China and the first time to detect Genotype 4 in the sewage. Results from this study indicate that the possibilities of sporadic infections of HEV should be emphasized because virus still has the possibility to be circulating in the sewage in China.
本研究旨在调查中国深圳污水样本中戊型肝炎病毒（HEV）的存在情况。于2015年5月至7月从152个污水处理厂采集污水样本，包括家畜污水、生活污水和处理后的污水。152份样本中有2份来自家畜污水处理厂的样本HEV呈阳性（1.32%）。对HEV的部分ORF2片段进行测序，并使用MEGA5.1构建系统发育树。Blast和系统发育分析表明，这两个序列均属于HEV基因型4。据我们所知，这是中国首次对废水中HEV进行分子特征研究，也是首次在污水中检测到基因型4。本研究结果表明，应重视戊型肝炎病毒散发性感染的可能性，因为该病毒在中国的污水中仍有可能传播。
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