Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430030, People's Republic of China.
J Nat Prod. 2013 Dec 27;76(12):2253-62. doi: 10.1021/np400600c. Epub 2013 Nov 20.
Fifteen new ent-kaurane diterpenoids, compounds 1-15, and two known analogues, 4-epi-henryine A (16) and leukamenin E (17), were isolated from the whole plants of Salvia cavaleriei. The structures of the new compounds were established by spectroscopic methods, and their absolute configurations were determined by electronic circular dichroism and single-crystal X-ray diffraction analyses with Cu Kα radiation. Compounds 1-15 were evaluated for their cytotoxicity against five human cancer cell lines, HL-60, SMMC-7721, A-549, MCF-7, and SW480, as well as the noncancerous Beas-2B cell line. Compounds 1-10, 12, 14, and 15 showed broad-spectrum cytotoxicity, with compounds 1, 3, 6-10, 12, and 15 exhibiting more potent cytotoxicity than the positive control, cis-platin, with IC50 values ranging from 0.65 to 6.4 μM.
从丹参全草中分离得到了 15 个新的贝壳杉烷二萜类化合物，化合物 1-15，以及两个已知类似物，4-epi-henryine A（16）和 leukamenin E（17）。通过光谱方法确定了新化合物的结构，并通过电子圆二色性和单晶 X 射线衍射分析用 Cu Kα 辐射确定了它们的绝对构型。对化合物 1-15 对五种人癌细胞系 HL-60、SMMC-7721、A-549、MCF-7 和 SW480 以及非癌细胞系 Beas-2B 的细胞毒性进行了评估。化合物 1-10、12、14 和 15 表现出广谱细胞毒性，化合物 1、3、6-10、12 和 15 的细胞毒性比阳性对照顺铂更强，IC50 值范围为 0.65 至 6.4 μM。
