Bruck C, Co M S, Slaoui M, Gaulton G N, Smith T, Fields B N, Mullins J I, Greene M I
Proc Natl Acad Sci U S A. 1986 Sep;83(17):6578-82. doi: 10.1073/pnas.83.17.6578.
The monoclonal anti-idiotypic antibody (mAb2) 87.92.6 directed against the 9B.G5 antibody specific for the virus neutralizing epitope on the mammalian reovirus type 3 hemagglutinin was previously demonstrated to express an internal image of the receptor binding epitope of the reovirus type 3. Furthermore, this mAb2 has autoimmune reactivity to the cell surface receptor of the reovirus. The nucleotide and deduced amino acid sequences of the 87.92.6 mAb2 heavy and light chains are described in this report. The sequence analysis reveals that the same heavy chain variable and joining (VH and JH) gene segments are used by the 87.92.6 anti-idiotypic mAb2 and by the dominant idiotypes of the BALB/c anti-GAT (cGAT) and anti-NP (NPa) responses. [GAT; random polymer that is 60% glutamic acid, 30% alanine, and 10% tyrosine. NP; (4-hydroxy-3-nitrophenyl)-acetyl.] Despite extensive homology at the level of the heavy chain variable regions, the NPa positive BALB/c anti-NP monoclonal antibody 17.2.25 binds neither 9B.G5 nor the cellular receptor for the hemagglutinin. Amino acid sequence comparison between the viral hemagglutinin and the 87.92.6 mAb2 light chain "internal image," reveals an area of significant homology indicating that antigen mimicry by antibodies may be achieved by sharing primary structure.
针对哺乳动物呼肠孤病毒3型血凝素上病毒中和表位的特异性9B.G5抗体的单克隆抗独特型抗体（mAb2）87.92.6，先前已证明其表达呼肠孤病毒3型受体结合表位的内影像。此外，该mAb2对呼肠孤病毒的细胞表面受体具有自身免疫反应性。本报告描述了87.92.6 mAb2重链和轻链的核苷酸及推导的氨基酸序列。序列分析显示，87.92.6抗独特型mAb2与BALB/c抗GAT（cGAT）和抗NP（NPa）反应的优势独特型使用相同的重链可变区和连接区（VH和JH）基因片段。[GAT；由60%谷氨酸、30%丙氨酸和10%酪氨酸组成的随机聚合物。NP；（4-羟基-3-硝基苯基）-乙酰基。]尽管在重链可变区水平存在广泛同源性，但NPa阳性的BALB/c抗NP单克隆抗体17.2.25既不结合9B.G5，也不结合血凝素的细胞受体。病毒血凝素与87.92.6 mAb2轻链“内影像”之间的氨基酸序列比较，揭示了一个显著同源区域，表明抗体的抗原模拟可能通过共享一级结构来实现。
