Mogal Vishal, Papper Vladislav, Chaurasia Alok, Feng Gao, Marks Robert, Steele Terry
Materials and Science Engineering, Division of Materials Technology, Nanyang Technological University, Singapore, 639798.
Macromol Biosci. 2014 Apr;14(4):478-84. doi: 10.1002/mabi.201300380. Epub 2013 Dec 2.
Current methods of tissue fixation rely on mechanical-related technologies developed from the clothing and carpentry industries. Herein, a novel bioadhesive method that allows tuneable adhesion and is also applicable to biodegradable polyester substrates is described. Diazirine is the key functional group that allows strong soft tissue crosslinking and on-demand adhesion based on a free radical mechanism. Plasma post-irradiation grafting makes it possible to graft diazirine onto PLGA substrates. When the diazirine-PLGA films, placed on wetted ex vivo swine aortas, are activated with low intensity UV light, lap shear strength of up to 450 ± 50 mN cm(-2) is observed, which is one order of magnitude higher than hydrogel bioadhesives placed on similar soft tissues. The diazirine-modified PLGA thin films could be added on top of previously developed technologies for minimally invasive surgeries. The present work is focused on the chemistry, grafting, and lap shear strength of the alkyl diazirine-modified PLGA bioadhesive films.
目前的组织固定方法依赖于源自服装和木工行业的机械相关技术。在此，描述了一种新型生物粘附方法，该方法具有可调节的粘附性，并且也适用于可生物降解的聚酯基材。重氮丙啶是关键官能团，它能基于自由基机制实现强软组织交联和按需粘附。等离子体后辐照接枝使得将重氮丙啶接枝到聚乳酸-羟基乙酸共聚物（PLGA）基材上成为可能。当置于湿的离体猪主动脉上的重氮丙啶-PLGA薄膜用低强度紫外光激活时，观察到搭接剪切强度高达450±50 mN cm(-2)，这比置于类似软组织上的水凝胶生物粘合剂高一个数量级。重氮丙啶改性的PLGA薄膜可添加到先前开发的微创手术技术之上。目前的工作集中在烷基重氮丙啶改性的PLGA生物粘附薄膜的化学性质、接枝和搭接剪切强度方面。
