School of Material Science and Engineering, Beijing University of Chemistry Technology, North Third Ring Road 15, Chaoyang District, Beijing 100029, China.
Escuela de Ingeniería y Ciencias, Tecnologico de Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey 64849, NL, Mexico.
Molecules. 2018 Mar 30;23(4):796. doi: 10.3390/molecules23040796.
Tissue adhesives based on polyamidoamine (PAMAM) dendrimer, grafted with UV-sensitive aryldiazirine (PAMAM-g-diazirine) are promising new candidates for light active adhesion on soft tissues. Diazirine carbene precursors form interfacial and intermolecular covalent crosslinks with tissues after UV light activation that requires no premixing or inclusion of free radical initiators. However, primary amines on the PAMAM dendrimer surface present a potential risk due to their cytotoxic and immunological effects. PAMAM-g-diazirine formulations with cationic pendant amines converted into neutral amide groups were evaluated. In vitro toxicity is reduced by an order of magnitude upon amine capping while retaining bioadhesive properties. The in vivo immunological response to PAMAM-g-diazirine formulations was found to be optimal in comparison to standard poly(lactic--glycolic acid) (PLGA) thin films.
基于聚酰胺-胺（PAMAM）树枝状大分子，接枝上紫外光敏感的重氮化合物（PAMAM-g-重氮化合物）的组织胶粘剂是软组织光活性黏附的有前途的新型候选物。重氮卡宾前体在紫外光激活后与组织形成界面和分子间共价交联，不需要预混合或自由基引发剂。然而，PAMAM 树枝状大分子表面上的伯胺由于其细胞毒性和免疫原性作用而存在潜在风险。评估了带有阳离子侧链伯胺的 PAMAM-g-重氮化合物制剂转化为中性酰胺基团。在保留生物黏附特性的同时，胺封端可使体外毒性降低一个数量级。与标准聚（乳酸-乙醇酸）（PLGA）薄膜相比，发现 PAMAM-g-重氮化合物制剂的体内免疫反应最佳。
