Nadort Annemarie, Woolthuis Rutger G, van Leeuwen Ton G, Faber Dirk J
Department of Biomedical Engineering and Physics, Academic Medical Center, University of Amsterdam, P.O. Box 22700, 1100 DE Amsterdam, The Netherlands ; MQ Biofocus Research Centre, Macquarie University, NSW, Sydney 2109, Australia.
Biomed Opt Express. 2013 Oct 7;4(11):2347-61. doi: 10.1364/BOE.4.002347. eCollection 2013.
We present integrated Laser Speckle Contrast Imaging (LSCI) and Sidestream Dark Field (SDF) flowmetry to provide real-time, non-invasive and quantitative measurements of speckle decorrelation times related to microcirculatory flow. Using a multi exposure acquisition scheme, precise speckle decorrelation times were obtained. Applying SDF-LSCI in vitro and in vivo allows direct comparison between speckle contrast decorrelation and flow velocities, while imaging the phantom and microcirculation architecture. This resulted in a novel analysis approach that distinguishes decorrelation due to flow from other additive decorrelation sources.
我们展示了集成激光散斑对比成像（LSCI）和侧流暗场（SDF）血流测量技术，以提供与微循环血流相关的散斑去相关时间的实时、非侵入性和定量测量。使用多曝光采集方案，获得了精确的散斑去相关时间。在体外和体内应用SDF-LSCI，可以在对体模和微循环结构成像的同时，直接比较散斑对比度去相关和流速。这产生了一种新颖的分析方法，可区分由血流引起的去相关与其他附加去相关源。
