Thiele H G, Koch F, Hamann A, Arndt R
Immunology. 1986 Oct;59(2):195-201.
This study presents the partial molecular characterization of the alloantigenic rat T-cell marker RT-6. In contrast to most lymphocyte surface membrane proteins, RT-6 proved to be resistant to short-term (15 min) solubilization by TX-100 at 4 degrees, but could be efficiently solubilized by long-term (16 hr) exposure at 4 degrees or short-term (15 min) exposure at 37 degrees. SDS-PAGE analyses of RT-6.2 immunoprecipitates under non-reducing conditions revealed two bands with apparent molecular weights of 21,000 and 24,000 (25,000 and 28,000 under reducing conditions). Radiolabelled sugars failed to be incorporated metabolically into RT-6.2, neither endoglycosidase-F and 0-glycanase digestion nor NaOH treatment caused any detectable decrease in the molecular weight of RT-6.2, and RT-6.2 failed to bind to any of a series of agarose-coupled lectins with a wide of sugar-binding specificities. These observations suggest that RT-6.2 may lack carbohydrate moieties.
本研究展示了同种异体抗原性大鼠T细胞标志物RT-6的部分分子特征。与大多数淋巴细胞表面膜蛋白不同，RT-6在4℃下经TX-100短期（15分钟）处理后仍具有抗性，但在4℃下长期（16小时）处理或在37℃下短期（15分钟）处理可使其有效溶解。在非还原条件下对RT-6.2免疫沉淀物进行SDS-PAGE分析，显示出两条带，表观分子量分别为21,000和24,000（在还原条件下为25,000和28,000）。放射性标记的糖类未能代谢性掺入RT-6.2中，内切糖苷酶-F和O-聚糖酶消化以及NaOH处理均未导致RT-6.2分子量出现任何可检测到的降低，并且RT-6.2未能与一系列具有广泛糖结合特异性的琼脂糖偶联凝集素中的任何一种结合。这些观察结果表明RT-6.2可能缺乏碳水化合物部分。
